Molecularly imprinted spongy columns for Angiotensin(II) recognition from human serum.
Biotechnol Prog
; 37(2): e3112, 2021 03.
Article
in English
| MEDLINE | ID: covidwho-1384129
ABSTRACT
Angiotensin II (AngII), the effector peptide of the renin angiotensin system and has an important role in regulating cardiovascular hemodynamics and structure. AngII is an important biomarker for certain diseases that are associated with cardiovascular disorders, i.e., influenza, SARS-CoV-2, tumors, hypertension, etc. However, AngII presents in blood in very low concentrations and they are not stable due to their reactivity, therefore spontaneous detection of AngII is a big challenge. In this study, AngII-imprinted spongy columns (AngII-misc) synthesized for AngII detection from human serum, and characterized by surface area measurements (BET), swelling tests, scanning electron microscopy (SEM), FTIR studies. AngII binding studies were achieved from aqueous environment and maximum binding capacity was found as 0.667 mg/g. It was calculated that the AngII-miscs recognized AngII 8.27 and 14.25 times more selectively than competitor Angiotensin I and Vasopressin molecules. Newly produced AngII-misc binds 60.5 pg/g AngII from crude human serum selectively. It has a great potential for spontaneous detection of AngII from human serum for direct and critical measurements in serious diseases, that is, heart attacks, SARS-CoV-2, etc.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Angiotensin II
/
Molecularly Imprinted Polymers
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Biotechnol Prog
Journal subject:
Biotechnology
Year:
2021
Document Type:
Article
Affiliation country:
BTPR.3112
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