Nucleotide-Bearing Benzylidene-Tetrahydroxanthylium Near-IR Fluorophore for Sensing DNA Replication, Secondary Structures and Interactions.
Chemistry
; 26(52): 11950-11954, 2020 Sep 16.
Article
in English
| MEDLINE | ID: covidwho-1384141
ABSTRACT
Thymidine triphosphate bearing benzylidene-tetrahydroxanthylium near-IR fluorophore linked to the 5-methyl group via triazole was synthesized through the CuAAC reaction and was used for polymerase synthesis of labelled DNA probes. The fluorophore lights up upon incorporation to DNA (up to 348-times) presumably due to interactions in major groove and the fluorescence further increases in the single-stranded oligonucleotide. The labelled dsDNA senses binding of small molecules and proteins by a strong decrease of fluorescence. The nucleotide was used as a light-up building block in real-time PCR for detection of SARS-CoV-2 virus.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
DNA Replication
/
COVID-19
Limits:
Humans
Language:
English
Journal:
Chemistry
Journal subject:
Chemistry
Year:
2020
Document Type:
Article
Affiliation country:
CHEM.202003192
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