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Self-sampling versus health care professional-guided swab collection for SARS-CoV-2 testing.
Würstle, Silvia; Spinner, Christoph D; Voit, Florian; Hoffmann, Dieter; Hering, Svenja; Weidlich, Simon; Schneider, Jochen; Zink, Alexander; Treiber, Matthias; Iakoubov, Roman; Schmid, Roland M; Protzer, Ulrike; Erber, Johanna.
  • Würstle S; Department of Internal Medicine II, School of Medicine, University Hospital Rechts der Isar, Technical University of Munich, Ismaninger Str. 22, 81675, Munich, Germany.
  • Spinner CD; Department of Internal Medicine II, School of Medicine, University Hospital Rechts der Isar, Technical University of Munich, Ismaninger Str. 22, 81675, Munich, Germany. christoph.spinner@tum.de.
  • Voit F; German Center for Infection Research (DZIF), Partner Site Munich, Munich, Germany. christoph.spinner@tum.de.
  • Hoffmann D; Department of Internal Medicine II, School of Medicine, University Hospital Rechts der Isar, Technical University of Munich, Ismaninger Str. 22, 81675, Munich, Germany.
  • Hering S; Institute of Virology, School of Medicine, Technical University of Munich/Helmholtz Zentrum München, Munich, Germany.
  • Weidlich S; Institute of Virology, School of Medicine, Technical University of Munich/Helmholtz Zentrum München, Munich, Germany.
  • Schneider J; Department of Internal Medicine II, School of Medicine, University Hospital Rechts der Isar, Technical University of Munich, Ismaninger Str. 22, 81675, Munich, Germany.
  • Zink A; German Center for Infection Research (DZIF), Partner Site Munich, Munich, Germany.
  • Treiber M; Department of Internal Medicine II, School of Medicine, University Hospital Rechts der Isar, Technical University of Munich, Ismaninger Str. 22, 81675, Munich, Germany.
  • Iakoubov R; German Center for Infection Research (DZIF), Partner Site Munich, Munich, Germany.
  • Schmid RM; Department of Dermatology and Allergology, School of Medicine, University Hospital Rechts der Isar, Technical University of Munich, Munich, Germany.
  • Protzer U; Department of Internal Medicine II, School of Medicine, University Hospital Rechts der Isar, Technical University of Munich, Ismaninger Str. 22, 81675, Munich, Germany.
  • Erber J; Department of Internal Medicine II, School of Medicine, University Hospital Rechts der Isar, Technical University of Munich, Ismaninger Str. 22, 81675, Munich, Germany.
Infection ; 49(5): 927-934, 2021 Oct.
Article in English | MEDLINE | ID: covidwho-1384715
ABSTRACT

PURPOSE:

To evaluate the diagnostic reliability and practicability of self-collected oropharyngeal swab samples for the detection of SARS-CoV-2 infection as self-sampling could enable broader testing availability and reduce both personal protective equipment and potential exposure.

METHODS:

Hospitalized SARS-CoV-2-infected patients were asked to collect two oropharyngeal swabs (SC-OPS1/2), and an additional oropharyngeal swab was collected by a health care professional (HCP-OPS). SARS-CoV-2 PCR testing for samples from 58 participants was performed, with a 48-h delay in half of the self-collected samples (SC-OPS2). The sensitivity, probability of concordance, and interrater reliability were calculated. Univariate and multivariate analyses were performed to assess predictive factors. Practicability was evaluated through a questionnaire.

RESULTS:

The test sensitivity for HCP-OPS, SC-OPS1, and SC-OPS2 was 88%, 78%, and 77%, respectively. Combining both SC-OPS results increased the estimated sensitivity to 88%. The concordance probability between HCP-OPS and SC-OPS1 was 77.6% and 82.5% between SC-OPS1 and SC-OPS2, respectively. Of the participants, 69% affirmed performing future self-sampling at home, and 34% preferred self-sampling over HCP-guided testing. Participants with both positive HCP-OPS1 and SC-OPS1 indicating no challenges during self-sampling had more differences in viral load levels between HCP-OPS1 and SC-OPS1 than those who indicated challenges. Increasing disease duration and the presence of anti-SARS-CoV-2-IgG correlated with negative test results in self-collected samples of previously confirmed SARS-CoV-2 positive individuals.

CONCLUSION:

Oropharyngeal self-sampling is an applicable testing approach for SARS-CoV-2 diagnostics. Self-sampling tends to be more effective in early versus late infection and symptom onset, and the collection of two distinct samples is recommended to maintain high test sensitivity.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Observational study / Prognostic study Topics: Long Covid Limits: Humans Language: English Journal: Infection Year: 2021 Document Type: Article Affiliation country: S15010-021-01614-9

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Observational study / Prognostic study Topics: Long Covid Limits: Humans Language: English Journal: Infection Year: 2021 Document Type: Article Affiliation country: S15010-021-01614-9