Glycomics-informed glycoproteomic analysis of site-specific glycosylation for SARS-CoV-2 spike protein.
STAR Protoc
; 1(3): 100214, 2020 12 18.
Article
in English
| MEDLINE | ID: covidwho-1386744
ABSTRACT
This protocol describes an integrated approach for analyzing site-specific N- and O-linked glycosylation of SARS-CoV-2 spike protein by mass spectrometry. Glycoproteomics analyzes intact glycopeptides to examine site-specific microheterogeneity of glycoproteins. Glycomics provides structural characterization on any glycan assignments by glycoproteomics. This procedure can be modified and applied to a variety of N- and/or O-linked glycoproteins. Combined with bioinformatics, the glycomics-informed glycoproteomics may be useful in generating 3D molecular dynamics simulations of certain glycoproteins alone or interacting with one another. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Glycoproteins
/
Proteomics
/
Glycomics
/
Spike Glycoprotein, Coronavirus
Limits:
Humans
Language:
English
Journal:
STAR Protoc
Year:
2020
Document Type:
Article
Affiliation country:
J.XPRO.2020.100214
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