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The specific metabolome profiling of patients infected by SARS-COV-2 supports the key role of tryptophan-nicotinamide pathway and cytosine metabolism.
Blasco, H; Bessy, C; Plantier, L; Lefevre, A; Piver, E; Bernard, L; Marlet, J; Stefic, K; Benz-de Bretagne, Isabelle; Cannet, P; Lumbu, H; Morel, T; Boulard, P; Andres, C R; Vourc'h, P; Hérault, O; Guillon, A; Emond, P.
  • Blasco H; UMR 1253, iBrain, équipe « neurogénomique et physiopathologie neuronale ¼, INSERM, Université de Tours, Tours, France. helene.blasco@univ-tours.fr.
  • Bessy C; Service de Biochimie et Biologie Moléculaire, CHU de Tours, Tours, France. helene.blasco@univ-tours.fr.
  • Plantier L; Service de Pneumologie et d'Explorations Fonctionnelles Respiratoires, CHU, Tours, France.
  • Lefevre A; Service de Pneumologie et d'Explorations Fonctionnelles Respiratoires, CHU, Tours, France.
  • Piver E; Centre d'Etude Des Pathologies Respiratoires, INSERM U1100, Université de Tours, Tours, France.
  • Bernard L; UMR 1253, iBrain, équipe « neurogénomique et physiopathologie neuronale ¼, INSERM, Université de Tours, Tours, France.
  • Marlet J; Service de Biochimie et Biologie Moléculaire, CHU de Tours, Tours, France.
  • Stefic K; INSERM U1259, MAVIVH, Université de Tours, Tours, France.
  • Benz-de Bretagne I; Service de Médecine Interne et Maladies Infectieuses, CHU de Tours, Tours, France.
  • Cannet P; INSERM U1259, MAVIVH, Université de Tours, Tours, France.
  • Lumbu H; Service de Bactériologie-Virologie-Hygiène, CHU de Tours, Tours, France.
  • Morel T; INSERM U1259, MAVIVH, Université de Tours, Tours, France.
  • Boulard P; Service de Bactériologie-Virologie-Hygiène, CHU de Tours, Tours, France.
  • Andres CR; UMR 1253, iBrain, équipe « neurogénomique et physiopathologie neuronale ¼, INSERM, Université de Tours, Tours, France.
  • Vourc'h P; Service de Biochimie et Biologie Moléculaire, CHU de Tours, Tours, France.
  • Hérault O; Service de Biochimie et Biologie Moléculaire, CHU de Tours, Tours, France.
  • Guillon A; Service de Biochimie et Biologie Moléculaire, CHU de Tours, Tours, France.
  • Emond P; Service de Biochimie et Biologie Moléculaire, CHU de Tours, Tours, France.
Sci Rep ; 10(1): 16824, 2020 10 08.
Article in English | MEDLINE | ID: covidwho-1387453
ABSTRACT
The biological mechanisms involved in SARS-CoV-2 infection are only partially understood. Thus we explored the plasma metabolome of patients infected with SARS-CoV-2 to search for diagnostic and/or prognostic biomarkers and to improve the knowledge of metabolic disturbance in this infection. We analyzed the plasma metabolome of 55 patients infected with SARS-CoV-2 and 45 controls by LC-HRMS at the time of viral diagnosis (D0). We first evaluated the ability to predict the diagnosis from the metabotype at D0 in an independent population. Next, we assessed the feasibility of predicting the disease evolution at the 7th and 15th day. Plasma metabolome allowed us to generate a discriminant multivariate model to predict the diagnosis of SARS-CoV-2 in an independent population (accuracy > 74%, sensitivity, specificity > 75%). We identified the role of the cytosine and tryptophan-nicotinamide pathways in this discrimination. However, metabolomic exploration modestly explained the disease evolution. Here, we present the first metabolomic study in SARS-CoV-2 patients which showed a high reliable prediction of early diagnosis. We have highlighted the role of the tryptophan-nicotinamide pathway clearly linked to inflammatory signals and microbiota, and the involvement of cytosine, previously described as a coordinator of cell metabolism in SARS-CoV-2. These findings could open new therapeutic perspectives as indirect targets.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Tryptophan / Niacinamide / Coronavirus Infections / Cytosine / Metabolome / Metabolomics / Betacoronavirus Type of study: Diagnostic study / Experimental Studies / Observational study / Prognostic study Limits: Aged / Female / Humans / Male / Middle aged Country/Region as subject: Europa Language: English Journal: Sci Rep Year: 2020 Document Type: Article Affiliation country: S41598-020-73966-5

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Tryptophan / Niacinamide / Coronavirus Infections / Cytosine / Metabolome / Metabolomics / Betacoronavirus Type of study: Diagnostic study / Experimental Studies / Observational study / Prognostic study Limits: Aged / Female / Humans / Male / Middle aged Country/Region as subject: Europa Language: English Journal: Sci Rep Year: 2020 Document Type: Article Affiliation country: S41598-020-73966-5