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Human embryonic stem cell-derived cardiomyocyte platform screens inhibitors of SARS-CoV-2 infection.
Williams, Thomas L; Colzani, Maria T; Macrae, Robyn G C; Robinson, Emma L; Bloor, Stuart; Greenwood, Edward J D; Zhan, Jun Ru; Strachan, Gregory; Kuc, Rhoda E; Nyimanu, Duuamene; Maguire, Janet J; Lehner, Paul J; Sinha, Sanjay; Davenport, Anthony P.
  • Williams TL; Experimental Medicine and Immunotherapeutics, University of Cambridge, Addenbrooke's Hospital, Cambridge, UK.
  • Colzani MT; Wellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK.
  • Macrae RGC; Experimental Medicine and Immunotherapeutics, University of Cambridge, Addenbrooke's Hospital, Cambridge, UK.
  • Robinson EL; Wellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK.
  • Bloor S; School of Medicine, Division of Cardiology, University of Colorado Denver, Anschutz Medical Campus, Aurora, CO, USA.
  • Greenwood EJD; Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK.
  • Zhan JR; Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK.
  • Strachan G; Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK.
  • Kuc RE; Wellcome Trust-MRC Institute of Metabolic Science, Metabolic Research Laboratories, Addenbrooke's Biomedical Campus, Cambridge, UK.
  • Nyimanu D; Experimental Medicine and Immunotherapeutics, University of Cambridge, Addenbrooke's Hospital, Cambridge, UK.
  • Maguire JJ; Experimental Medicine and Immunotherapeutics, University of Cambridge, Addenbrooke's Hospital, Cambridge, UK.
  • Lehner PJ; Experimental Medicine and Immunotherapeutics, University of Cambridge, Addenbrooke's Hospital, Cambridge, UK.
  • Sinha S; Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK.
  • Davenport AP; Wellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK. ss661@cam.ac.uk.
Commun Biol ; 4(1): 926, 2021 07 29.
Article in English | MEDLINE | ID: covidwho-1387497
ABSTRACT
Patients with cardiovascular comorbidities are more susceptible to severe infection with SARS-CoV-2, known to directly cause pathological damage to cardiovascular tissue. We outline a screening platform using human embryonic stem cell-derived cardiomyocytes, confirmed to express the protein machinery critical for SARS-CoV-2 infection, and a SARS-CoV-2 spike-pseudotyped virus system. The method has allowed us to identify benztropine and DX600 as novel inhibitors of SARS-CoV-2 infection in a clinically relevant stem cell-derived cardiomyocyte line. Discovery of new medicines will be critical for protecting the heart in patients with SARS-CoV-2, and for individuals where vaccination is contraindicated.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Myocytes, Cardiac / Drug Evaluation, Preclinical / Human Embryonic Stem Cells / SARS-CoV-2 Type of study: Prognostic study Topics: Traditional medicine / Vaccines Limits: Humans Language: English Journal: Commun Biol Year: 2021 Document Type: Article Affiliation country: S42003-021-02453-Y

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Myocytes, Cardiac / Drug Evaluation, Preclinical / Human Embryonic Stem Cells / SARS-CoV-2 Type of study: Prognostic study Topics: Traditional medicine / Vaccines Limits: Humans Language: English Journal: Commun Biol Year: 2021 Document Type: Article Affiliation country: S42003-021-02453-Y