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Bronchoalveolar lavage affects thorax computed tomography of healthy and SARS-CoV-2 infected rhesus macaques (Macaca mulatta).
Maaskant, Annemiek; Meijer, Lisette; Fagrouch, Zahra; Bakker, Jaco; van Geest, Leo; Zijlmans, Dian G M; Verstrepen, Babs E; Langermans, Jan A M; Verschoor, Ernst J; Stammes, Marieke A.
  • Maaskant A; Biomedical Primate Research Centre (BPRC), Rijswijk, Netherlands.
  • Meijer L; Biomedical Primate Research Centre (BPRC), Rijswijk, Netherlands.
  • Fagrouch Z; Biomedical Primate Research Centre (BPRC), Rijswijk, Netherlands.
  • Bakker J; Biomedical Primate Research Centre (BPRC), Rijswijk, Netherlands.
  • van Geest L; Biomedical Primate Research Centre (BPRC), Rijswijk, Netherlands.
  • Zijlmans DGM; Biomedical Primate Research Centre (BPRC), Rijswijk, Netherlands.
  • Verstrepen BE; Biomedical Primate Research Centre (BPRC), Rijswijk, Netherlands.
  • Langermans JAM; Biomedical Primate Research Centre (BPRC), Rijswijk, Netherlands.
  • Verschoor EJ; Department Population Health Sciences, Division Animals in Science and Society, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands.
  • Stammes MA; Biomedical Primate Research Centre (BPRC), Rijswijk, Netherlands.
PLoS One ; 16(7): e0252941, 2021.
Article in English | MEDLINE | ID: covidwho-1388922
Preprint
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ABSTRACT
Medical imaging as method to assess the longitudinal process of a SARS-CoV-2 infection in non-human primates is commonly used in research settings. Bronchoalveolar lavage (BAL) is regularly used to determine the local virus production and immune effects of SARS-CoV-2 in the lower respiratory tract. However, the potential interference of those two diagnostic modalities is unknown in non-human primates. The current study investigated the effect and duration of BAL on computed tomography (CT) in both healthy and experimentally SARS-CoV-2-infected female rhesus macaques (Macaca mulatta). In addition, the effect of subsequent BALs was reviewed. Thorax CTs and BALs were obtained from four healthy animals and 11 experimentally SARS-CoV-2-infected animals. From all animals, CTs were obtained just before BAL, and 24 hours post-BAL. Additionally, from the healthy animals, CTs immediately after, and four hours post-BAL were obtained. Thorax CTs were evaluated for alterations in lung density, measured in Hounsfield units, and a visual semi-quantitative scoring system. An increase in the lung density was observed on the immediately post-BAL CT but resolved within 24 hours in the healthy animals. In the infected animals, a significant difference in both the lung density and CT score was still found 24 hours after BAL. Furthermore, the differences between time points in CT score were increased for the second BAL. These results indicate that the effect of BAL on infected lungs is not resolved within the first 24 hours. Therefore, it is important to acknowledge the interference between BAL and CT in rhesus macaques.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Tomography, X-Ray Computed / SARS-CoV-2 / COVID-19 / Lung Type of study: Experimental Studies / Prognostic study Limits: Animals Language: English Journal: PLoS One Journal subject: Science / Medicine Year: 2021 Document Type: Article Affiliation country: JOURNAL.PONE.0252941

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Tomography, X-Ray Computed / SARS-CoV-2 / COVID-19 / Lung Type of study: Experimental Studies / Prognostic study Limits: Animals Language: English Journal: PLoS One Journal subject: Science / Medicine Year: 2021 Document Type: Article Affiliation country: JOURNAL.PONE.0252941