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SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay.
Buck, Michael D; Poirier, Enzo Z; Cardoso, Ana; Frederico, Bruno; Canton, Johnathan; Barrell, Sam; Beale, Rupert; Byrne, Richard; Caidan, Simon; Crawford, Margaret; Cubitt, Laura; Gandhi, Sonia; Goldstone, Robert; Grant, Paul R; Gulati, Kiran; Hindmarsh, Steve; Howell, Michael; Hubank, Michael; Instrell, Rachael; Jiang, Ming; Kassiotis, George; Lu, Wei-Ting; MacRae, James I; Martini, Iana; Miller, Davin; Moore, David; Nastouli, Eleni; Nicod, Jerome; Nightingale, Luke; Olsen, Jessica; Oomatia, Amin; O'Reilly, Nicola; Rideg, Anett; Song, Ok-Ryul; Strange, Amy; Swanton, Charles; Turajlic, Samra; Wu, Mary; Reis e Sousa, Caetano.
  • Buck MD; The Francis Crick Institute, London, NW1 1AT, UK.
  • Poirier EZ; The Francis Crick Institute, London, NW1 1AT, UK.
  • Cardoso A; The Francis Crick Institute, London, NW1 1AT, UK.
  • Frederico B; The Francis Crick Institute, London, NW1 1AT, UK.
  • Canton J; The Francis Crick Institute, London, NW1 1AT, UK.
  • Barrell S; The Francis Crick Institute, London, NW1 1AT, UK.
  • Beale R; The Francis Crick Institute, London, NW1 1AT, UK.
  • Byrne R; The Francis Crick Institute, London, NW1 1AT, UK.
  • Caidan S; The Francis Crick Institute, London, NW1 1AT, UK.
  • Crawford M; The Francis Crick Institute, London, NW1 1AT, UK.
  • Cubitt L; The Francis Crick Institute, London, NW1 1AT, UK.
  • Gandhi S; The Francis Crick Institute, London, NW1 1AT, UK.
  • Goldstone R; University College London, London, WC1E 6BT, UK.
  • Grant PR; University College London Hospitals NHS Trust, London, NW1 2PG, UK.
  • Gulati K; The Francis Crick Institute, London, NW1 1AT, UK.
  • Hindmarsh S; Healh Service Laboratories, London, WC1H 9AX, UK.
  • Howell M; New England Biolabs, Ipswich, MA, USA.
  • Hubank M; The Francis Crick Institute, London, NW1 1AT, UK.
  • Instrell R; The Francis Crick Institute, London, NW1 1AT, UK.
  • Jiang M; The Royal Marsden Hospital, Sutton, SM2 5NG, UK.
  • Kassiotis G; The Institute of Cancer Research, London, SW7 3RP, UK.
  • Lu WT; The Francis Crick Institute, London, NW1 1AT, UK.
  • MacRae JI; The Francis Crick Institute, London, NW1 1AT, UK.
  • Martini I; The Francis Crick Institute, London, NW1 1AT, UK.
  • Miller D; The Francis Crick Institute, London, NW1 1AT, UK.
  • Moore D; The Francis Crick Institute, London, NW1 1AT, UK.
  • Nastouli E; The Royal Free Hospital, London, NW3 2QG, UK.
  • Nicod J; New England Biolabs, Ipswich, MA, USA.
  • Nightingale L; University College London, London, WC1E 6BT, UK.
  • Olsen J; University College London Hospitals NHS Trust, London, NW1 2PG, UK.
  • Oomatia A; The Francis Crick Institute, London, NW1 1AT, UK.
  • O'Reilly N; University College London Hospitals NHS Trust, London, NW1 2PG, UK.
  • Rideg A; University College London GOS Institute of Child Health, London, WC1N 1EH, UK.
  • Song OR; The Francis Crick Institute, London, NW1 1AT, UK.
  • Strange A; The Francis Crick Institute, London, NW1 1AT, UK.
  • Swanton C; The Francis Crick Institute, London, NW1 1AT, UK.
  • Turajlic S; The Royal Free Hospital, London, NW3 2QG, UK.
  • Wu M; The Francis Crick Institute, London, NW1 1AT, UK.
  • Reis e Sousa C; The Francis Crick Institute, London, NW1 1AT, UK.
Wellcome Open Res ; 6: 9, 2021.
Article in English | MEDLINE | ID: covidwho-1389814
ABSTRACT
The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority of patient samples is assessed for viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals and an accredited clinical diagnostic laboratory, established a diagnostic testing pipeline that has reported on more than 252,000 RT-qPCR results since its commencement at the beginning of April 2020. However, due to ongoing demand and competition for critical resources, alternative testing strategies were sought. In this work, we present a clinically-validated procedure for high-throughput SARS-CoV-2 detection by RT-LAMP in 25 minutes that is robust, reliable, repeatable, sensitive, specific, and inexpensive.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Prognostic study Language: English Journal: Wellcome Open Res Year: 2021 Document Type: Article Affiliation country: WELLCOMEOPENRES.16517.2

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Prognostic study Language: English Journal: Wellcome Open Res Year: 2021 Document Type: Article Affiliation country: WELLCOMEOPENRES.16517.2