Your browser doesn't support javascript.
Electrochemical detection of carbendazim with mulberry fruit-like gold nanocrystal/multiple graphene aerogel and DNA cycle amplification.
Jin, Wang; Ruiyi, Li; Nana, Li; Xiulan, Sun; Haiyan, Zhu; Guangli, Wang; Zaijun, Li.
  • Jin W; School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China.
  • Ruiyi L; School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, China.
  • Nana L; School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China.
  • Xiulan S; School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
  • Haiyan Z; School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China.
  • Guangli W; School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China.
  • Zaijun L; School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China. zaijunli@jiangnan.edu.cn.
Mikrochim Acta ; 188(8): 284, 2021 08 02.
Article in English | MEDLINE | ID: covidwho-1397013
ABSTRACT
An aptasensor for electrochemical detection of carbendazim is reported with mulberry fruit-like gold nanocrystal (MF-Au)/multiple graphene aerogel (MGA) and DNA cycle amplification. HAuCl4 was reduced by ascorbic acid in a CTAC solution containing KBr and KI and formed trioctahedron gold nanocrystal. The gold nanocrystal underwent structural evolution under enantioselective direction of L-cysteine. The resulting MF-Au shows a mulberry fruit-like nanostructure composed of gold nanocrystals of about 200 nm as the core and many irregular gold nanoparticles of about 30 nm as the shell. The exposure of high-index facets improves the catalytic activity of MF-Au. MF-Au/MGA was used for the construction of an aptasensor for electrochemical detection of carbendazim. The aptamer hybridizes with assistant strand DNA to form duplex DNA. Carbendazim binds with the formed duplex DNA to release assistant strand DNA, triggering one three-cascade DNA cycle. The utilization of a DNA cycle allows one carbendazim molecule to bring many methylene blue-labeled DNA fragments to the electrode surface. This promotes significant signal amplification due to the redox reaction of methylene blue. The detection signal is further enhanced by the catalysis of MF-Au and MGA towards the redox of methylene blue. A differential pulse voltammetric signal, best measured at - 0.32 V vs. Ag/AgCl, increases linearly with the carbendazim concentration ranging from 1.0 × 10-16 to 1.0 × 10-11 M with a detection limit of 4.4 × 10-17 M. The method provides ultrahigh sensitivity and selectivity and was successfully applied to the electrochemical detection of carbendazim in cucumber. This study reports on an ultrasensitive aptasensor for electrochemical detection of carbendazim in cucumber based on mulberry fruit-like gold nanocrystal-multiple graphene aerogel and DNA cycle double amplification.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Benzimidazoles / DNA / Carbamates / Biosensing Techniques / Aptamers, Nucleotide / Metal Nanoparticles Type of study: Diagnostic study Topics: Variants Language: English Journal: Mikrochim Acta Year: 2021 Document Type: Article Affiliation country: S00604-021-04886-y

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Benzimidazoles / DNA / Carbamates / Biosensing Techniques / Aptamers, Nucleotide / Metal Nanoparticles Type of study: Diagnostic study Topics: Variants Language: English Journal: Mikrochim Acta Year: 2021 Document Type: Article Affiliation country: S00604-021-04886-y