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Air-liquid interphase culture confers SARS-CoV-2 susceptibility to A549 alveolar epithelial cells.
Sasaki, Michihito; Kishimoto, Mai; Itakura, Yukari; Tabata, Koshiro; Intaruck, Kittiya; Uemura, Kentaro; Toba, Shinsuke; Sanaki, Takao; Sato, Akihiko; Hall, William W; Orba, Yasuko; Sawa, Hirofumi.
  • Sasaki M; Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan. Electronic address: m-sasaki@czc.hokudai.ac.jp.
  • Kishimoto M; Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan.
  • Itakura Y; Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan.
  • Tabata K; Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan.
  • Intaruck K; Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan.
  • Uemura K; Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan; Shionogi & Co., Ltd., Osaka, 541-0045, Japan; Laboratory of Biomolecular Science, Faculty of Pharmaceutical Science, Hokkaido University, Sapporo, 060-0812, Japan.
  • Toba S; Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan; Shionogi & Co., Ltd., Osaka, 541-0045, Japan.
  • Sanaki T; Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan; Shionogi & Co., Ltd., Osaka, 541-0045, Japan.
  • Sato A; Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan; Shionogi & Co., Ltd., Osaka, 541-0045, Japan.
  • Hall WW; International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan; National Virus Reference Laboratory, University College Dublin, Belfield, Dublin, 4, Ireland; Global Virus Network, Baltimore, MD, 21201, USA.
  • Orba Y; Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan; International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan.
  • Sawa H; Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan; International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan; Global Virus Network, Baltimore, MD, 21201,
Biochem Biophys Res Commun ; 577: 146-151, 2021 11 05.
Article in English | MEDLINE | ID: covidwho-1401239
ABSTRACT
The human lung cell A549 is susceptible to infection with a number of respiratory viruses. However, A549 cells are resistant to Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) infection in conventional submerged culture, and this would appear to be due to low expression levels of the SARS-CoV-2 entry receptor angiotensin-converting enzyme-2 (ACE2). Here, we examined SARS-CoV-2 susceptibility to A549 cells after adaptation to air-liquid interface (ALI) culture. A549 cells in ALI culture yielded a layer of mucus on their apical surface, exhibited decreased expression levels of the proliferation marker KI-67 and intriguingly became susceptible to SARS-CoV-2 infection. We found that A549 cells increased the endogenous expression levels of ACE2 and TMPRSS2 following adaptation to ALI culture conditions. Camostat, a TMPRSS2 inhibitor, reduced SARS-CoV-2 infection in ALI-cultured A549 cells. These findings indicate that ALI culture switches the phenotype of A549 cells from resistance to susceptibility to SARS-CoV-2 infection through upregulation of ACE2 and TMPRSS2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Cell Culture Techniques / Alveolar Epithelial Cells / SARS-CoV-2 / COVID-19 Limits: Humans Language: English Journal: Biochem Biophys Res Commun Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Cell Culture Techniques / Alveolar Epithelial Cells / SARS-CoV-2 / COVID-19 Limits: Humans Language: English Journal: Biochem Biophys Res Commun Year: 2021 Document Type: Article