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Comparison of FTD SARS-CoV-2 Assay and RealStar RT-PCR kit 1.0 for the detection of SARS-CoV-2.
Di Carlo, Daniele; Mazzuti, Laura; Sciandra, Ilaria; Guerrizio, Giuliana; Oliveto, Giuseppe; Riveros Cabral, Rodolfo J; Zingaropoli, Maria Antonella; Turriziani, Ombretta.
  • Di Carlo D; Department of Molecular Medicine, Sapienza University, Viale dell'Università 31, 00185, Rome, Italy.
  • Mazzuti L; Department of Molecular Medicine, Sapienza University, Viale dell'Università 31, 00185, Rome, Italy.
  • Sciandra I; Department of Molecular Medicine, Sapienza University, Viale dell'Università 31, 00185, Rome, Italy.
  • Guerrizio G; Department of Molecular Medicine, Sapienza University, Viale dell'Università 31, 00185, Rome, Italy.
  • Oliveto G; Department of Molecular Medicine, Sapienza University, Viale dell'Università 31, 00185, Rome, Italy.
  • Riveros Cabral RJ; Department of Molecular Medicine, Sapienza University, Viale dell'Università 31, 00185, Rome, Italy.
  • Zingaropoli MA; Department of Public Health and Infectious Diseases, Sapienza University, Viale dell'Università 31, 00185, Rome, Italy.
  • Turriziani O; Department of Molecular Medicine, Sapienza University, Viale dell'Università 31, 00185, Rome, Italy. Electronic address: ombretta.turriziani@uniroma1.it.
J Virol Methods ; 298: 114276, 2021 12.
Article in English | MEDLINE | ID: covidwho-1401673
ABSTRACT
The aim of the study was to evaluate the clinical performance of FTD SARS-CoV-2 compared to the RealStar RT-PCR kit 1.0. The analysis of 100 nasopharyngeal swabs showed an overall agreement of 88 %. The positive percentage agreement was 85.6 % and the negative percentage agreement was 91 %. In conclusion we observed a substantial agreement among the two methods, with discrepancies mainly observed in specimens with relatively low amount of viral RNA.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Frontotemporal Dementia / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: J Virol Methods Year: 2021 Document Type: Article Affiliation country: J.jviromet.2021.114276

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Frontotemporal Dementia / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: J Virol Methods Year: 2021 Document Type: Article Affiliation country: J.jviromet.2021.114276