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A new flow cytometry assay to measure antibody-dependent cellular cytotoxicity against SARS-CoV-2 Spike-expressing cells.
Beaudoin-Bussières, Guillaume; Richard, Jonathan; Prévost, Jérémie; Goyette, Guillaume; Finzi, Andrés.
  • Beaudoin-Bussières G; Centre de Recherche du CHUM, Montréal, QC H2X 0A9, Canada.
  • Richard J; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, QC H3T 1J4, Canada.
  • Prévost J; Centre de Recherche du CHUM, Montréal, QC H2X 0A9, Canada.
  • Goyette G; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, QC H3T 1J4, Canada.
  • Finzi A; Centre de Recherche du CHUM, Montréal, QC H2X 0A9, Canada.
STAR Protoc ; 2(4): 100851, 2021 12 17.
Article in English | MEDLINE | ID: covidwho-1401935
ABSTRACT
Antibodies can engage specific receptors at the surface of effector cells and mediate several functions beyond viral neutralization. Increasing evidence suggests that Fc-mediated effector functions, such as antibody-dependent cellular cytotoxicity (ADCC), have an important role in protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. We engineered a cell line stably expressing a GFP-tagged SARS-CoV-2 spike to measure ADCC. This protocol provides an optimized way of measuring ADCC activity mediated by anti-SARS-CoV-2 Spike monoclonal antibodies or plasma from previously infected or vaccinated individuals. For complete details on the use and execution of this protocol, please refer to Anand et al. (2021b).
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antibodies, Neutralizing / Flow Cytometry / SARS-CoV-2 / COVID-19 / Antibodies, Monoclonal / Antibodies, Viral / Antibody-Dependent Cell Cytotoxicity Topics: Vaccines Limits: Humans Language: English Journal: STAR Protoc Year: 2021 Document Type: Article Affiliation country: J.xpro.2021.100851

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antibodies, Neutralizing / Flow Cytometry / SARS-CoV-2 / COVID-19 / Antibodies, Monoclonal / Antibodies, Viral / Antibody-Dependent Cell Cytotoxicity Topics: Vaccines Limits: Humans Language: English Journal: STAR Protoc Year: 2021 Document Type: Article Affiliation country: J.xpro.2021.100851