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Application of the amplification-free SERS-based CRISPR/Cas12a platform in the identification of SARS-CoV-2 from clinical samples.
Liang, Jiajie; Teng, Peijun; Xiao, Wei; He, Guanbo; Song, Qifang; Zhang, Ying; Peng, Bin; Li, Gan; Hu, Liangshan; Cao, Donglin; Tang, Yong.
  • Liang J; Department of Bioengineering, Guangdong Province Engineering Research Center of Antibody Drug and Immunoassay, College of Life Science and Technology, Jinan University, Guangzhou, 510632, People's Republic of China.
  • Teng P; Guangdong Biowings Tech Limited, Foshan, 528000, People's Republic of China.
  • Xiao W; Department of Bioengineering, Guangdong Province Engineering Research Center of Antibody Drug and Immunoassay, College of Life Science and Technology, Jinan University, Guangzhou, 510632, People's Republic of China.
  • He G; Department of Laboratory Medicine, Guangdong Second Provincial General Hospital, Guangzhou, 510317, People's Republic of China.
  • Song Q; Guangdong Biowings Tech Limited, Foshan, 528000, People's Republic of China.
  • Zhang Y; Department of Bioengineering, Guangdong Province Engineering Research Center of Antibody Drug and Immunoassay, College of Life Science and Technology, Jinan University, Guangzhou, 510632, People's Republic of China.
  • Peng B; Department of Bioengineering, Guangdong Province Engineering Research Center of Antibody Drug and Immunoassay, College of Life Science and Technology, Jinan University, Guangzhou, 510632, People's Republic of China.
  • Li G; Department of Bioengineering, Guangdong Province Engineering Research Center of Antibody Drug and Immunoassay, College of Life Science and Technology, Jinan University, Guangzhou, 510632, People's Republic of China.
  • Hu L; Department of Bioengineering, Guangdong Province Engineering Research Center of Antibody Drug and Immunoassay, College of Life Science and Technology, Jinan University, Guangzhou, 510632, People's Republic of China.
  • Cao D; Department of Laboratory Medicine, Guangdong Second Provincial General Hospital, Guangzhou, 510317, People's Republic of China. liangshan8027@163.com.
  • Tang Y; Department of Laboratory Medicine, Guangdong Second Provincial General Hospital, Guangzhou, 510317, People's Republic of China. caodl@126.com.
J Nanobiotechnology ; 19(1): 273, 2021 Sep 08.
Article in English | MEDLINE | ID: covidwho-1403239
ABSTRACT
The control of contagious or refractory diseases requires early, rapid diagnostic assays that are simple, fast, and easy-to-use. Here, easy-to-implement CRISPR/Cas12a-based diagnostic platform through Raman transducer generated by Raman enhancement effect, term as SERS-CRISPR (S-CRISPR), are described. The S-CRISPR uses high-activity noble metallic nanoscopic materials to increase the sensitivity in the detection of nucleic acids, without amplification. This amplification-free platform, which can be performed within 30-40 min of incubation time, is then used for detection of SARS-CoV-2 derived nucleic acids in RNA extracts obtained from nasopharyngeal swab specimens (n = 112). Compared with the quantitative reverse transcription polymerase chain reaction (RT-qPCR), the sensitivity and specificity of S-CRISPR reaches 87.50% and 100%, respectively. In general, the S-CRISPR can rapidly identify the RNA of SARS-CoV-2 RNA without amplification and is a potential strategy for nucleic acid point of care test (POCT).
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Spectrum Analysis, Raman / Nucleic Acid Amplification Techniques / CRISPR-Cas Systems / SARS-CoV-2 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: J Nanobiotechnology Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Spectrum Analysis, Raman / Nucleic Acid Amplification Techniques / CRISPR-Cas Systems / SARS-CoV-2 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: J Nanobiotechnology Year: 2021 Document Type: Article