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Platinum chloride-based viability RT-qPCR for SARS-CoV-2 detection in complex samples.
Cuevas-Ferrando, Enric; Randazzo, Walter; Pérez-Cataluña, Alba; Falcó, Irene; Navarro, David; Martin-Latil, Sandra; Díaz-Reolid, Azahara; Girón-Guzmán, Inés; Allende, Ana; Sánchez, Gloria.
  • Cuevas-Ferrando E; Department of Preservation and Food Safety Technologies, Institute of Agrochemistry and Food Technology, IATA-CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain. enric.cuevas@iata.csic.es.
  • Randazzo W; Department of Preservation and Food Safety Technologies, Institute of Agrochemistry and Food Technology, IATA-CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain.
  • Pérez-Cataluña A; Department of Preservation and Food Safety Technologies, Institute of Agrochemistry and Food Technology, IATA-CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain.
  • Falcó I; Department of Preservation and Food Safety Technologies, Institute of Agrochemistry and Food Technology, IATA-CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain.
  • Navarro D; Microbiology Service, Clinic University Hospital, INCLIVA Health Research Institute, Valencia, Spain.
  • Martin-Latil S; Department of Microbiology, School of Medicine, University of Valencia, Valencia, Spain.
  • Díaz-Reolid A; ANSES Laboratory for Food Safety, Université Paris-Est, 94700, Maisons-Alfort, France.
  • Girón-Guzmán I; Department of Preservation and Food Safety Technologies, Institute of Agrochemistry and Food Technology, IATA-CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain.
  • Allende A; Department of Preservation and Food Safety Technologies, Institute of Agrochemistry and Food Technology, IATA-CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain.
  • Sánchez G; Research Group on Quality and Safety of Fruits and Vegetables, Department of Food Science and Technology, CEBAS-CSIC, Campus Universitario de Espinardo, 25, 30100, Murcia, Spain.
Sci Rep ; 11(1): 18120, 2021 09 13.
Article in English | MEDLINE | ID: covidwho-1406410
ABSTRACT
Isolation, contact tracing and restrictions on social movement are being globally implemented to prevent and control onward spread of SARS-CoV-2, even though the infection risk modelled on RNA detection by RT-qPCR remains biased as viral shedding and infectivity are not discerned. Thus, we aimed to develop a rapid viability RT-qPCR procedure to infer SARS-CoV-2 infectivity in clinical specimens and environmental samples. We screened monoazide dyes and platinum compounds as viability molecular markers on five SARS-CoV-2 RNA targets. A platinum chloride-based viability RT-qPCR was then optimized using genomic RNA, and inactivated SARS-CoV-2 particles inoculated in buffer, stool, and urine. Our results were finally validated in nasopharyngeal swabs from persons who tested positive for COVID-19 and in wastewater samples positive for SARS-CoV-2 RNA. We established a rapid viability RT-qPCR that selectively detects potentially infectious SARS-CoV-2 particles in complex matrices. In particular, the confirmed positivity of nasopharyngeal swabs following the viability procedure suggests their potential infectivity, while the complete prevention of amplification in wastewater indicated either non-infectious particles or free RNA. The viability RT-qPCR approach provides a more accurate ascertainment of the infectious viruses detection and it may complement analyses to foster risk-based investigations for the prevention and control of new or re-occurring outbreaks with a broad application spectrum.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Platinum Compounds / Reverse Transcriptase Polymerase Chain Reaction / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Prognostic study Limits: Humans Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-97700-x

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Platinum Compounds / Reverse Transcriptase Polymerase Chain Reaction / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Prognostic study Limits: Humans Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-97700-x