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Development and preclinical evaluation of virus-like particle vaccine against COVID-19 infection.
Yilmaz, Ismail Cem; Ipekoglu, Emre Mert; Bulbul, Artun; Turay, Nilsu; Yildirim, Muzaffer; Evcili, Irem; Yilmaz, Naz Surucu; Guvencli, Nese; Aydin, Yagmur; Gungor, Bilgi; Saraydar, Berfu; Bartan, Asli Gulce; Ibibik, Bilgehan; Bildik, Tugce; Baydemir, Ilayda; Sanli, Hatice Asena; Kayaoglu, Basak; Ceylan, Yasemin; Yildirim, Tugce; Abras, Irem; Ayanoglu, Ihsan Cihan; Cam, Sefa Burak; Ciftci Dede, Eda; Gizer, Merve; Erganis, Osman; Sarac, Fahriye; Uzar, Serdar; Enul, Hakan; Adiay, Cumhur; Aykut, Gamze; Polat, Hivda; Yildirim, Ismail Selim; Tekin, Saban; Korukluoglu, Gulay; Zeytin, Hasan Ersin; Korkusuz, Petek; Gursel, Ihsan; Gursel, Mayda.
  • Yilmaz IC; Department of Biological Sciences, Middle East Technical University, Ankara, Turkey.
  • Ipekoglu EM; Molecular Biology and Genetics Department, Bilkent University, Ankara, Turkey.
  • Bulbul A; Department of Biological Sciences, Middle East Technical University, Ankara, Turkey.
  • Turay N; Molecular Biology and Genetics Department, Bilkent University, Ankara, Turkey.
  • Yildirim M; Molecular Biology and Genetics Department, Bilkent University, Ankara, Turkey.
  • Evcili I; Molecular Biology and Genetics Department, Bilkent University, Ankara, Turkey.
  • Yilmaz NS; Molecular Biology and Genetics Department, Bilkent University, Ankara, Turkey.
  • Guvencli N; Department of Biological Sciences, Middle East Technical University, Ankara, Turkey.
  • Aydin Y; Department of Biological Sciences, Middle East Technical University, Ankara, Turkey.
  • Gungor B; Department of Biological Sciences, Middle East Technical University, Ankara, Turkey.
  • Saraydar B; Molecular Biology and Genetics Department, Bilkent University, Ankara, Turkey.
  • Bartan AG; Molecular Biology and Genetics Department, Bilkent University, Ankara, Turkey.
  • Ibibik B; Molecular Biology and Genetics Department, Bilkent University, Ankara, Turkey.
  • Bildik T; Molecular Biology and Genetics Department, Bilkent University, Ankara, Turkey.
  • Baydemir I; Molecular Biology and Genetics Department, Bilkent University, Ankara, Turkey.
  • Sanli HA; Department of Biological Sciences, Middle East Technical University, Ankara, Turkey.
  • Kayaoglu B; Department of Biological Sciences, Middle East Technical University, Ankara, Turkey.
  • Ceylan Y; Department of Biological Sciences, Middle East Technical University, Ankara, Turkey.
  • Yildirim T; Molecular Biology and Genetics Department, Bilkent University, Ankara, Turkey.
  • Abras I; Molecular Biology and Genetics Department, Bilkent University, Ankara, Turkey.
  • Ayanoglu IC; Molecular Biology and Genetics Department, Bilkent University, Ankara, Turkey.
  • Cam SB; Department of Biological Sciences, Middle East Technical University, Ankara, Turkey.
  • Ciftci Dede E; Hacettepe University Faculty of Medicine Department of Histology and Embryology, Ankara, Turkey.
  • Gizer M; Hacettepe University Graduate School of Science and Engineering, Department of Bioengineering, Ankara, Turkey.
  • Erganis O; Hacettepe University Graduate School of Health Sciences, Department of Stem Cell Sciences, Ankara, Turkey.
  • Sarac F; Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey.
  • Uzar S; Pendik Veterinary Research and Control Institute, Istanbul, Turkey.
  • Enul H; Pendik Veterinary Research and Control Institute, Istanbul, Turkey.
  • Adiay C; Pendik Veterinary Research and Control Institute, Istanbul, Turkey.
  • Aykut G; Pendik Veterinary Research and Control Institute, Istanbul, Turkey.
  • Polat H; Molecular Biology and Genetics Department, Bilkent University, Ankara, Turkey.
  • Yildirim IS; Marmara Research Center, TUBITAK, Istanbul, Turkey.
  • Tekin S; Marmara Research Center, TUBITAK, Istanbul, Turkey.
  • Korukluoglu G; Marmara Research Center, TUBITAK, Istanbul, Turkey.
  • Zeytin HE; Virology Laboratory, General Directorate of Public Health, Ankara, Turkey.
  • Korkusuz P; Biotechnology Research Center, Nobel Pharma, Istanbul, Turkey.
  • Gursel I; Hacettepe University Faculty of Medicine Department of Histology and Embryology, Ankara, Turkey.
  • Gursel M; Molecular Biology and Genetics Department, Bilkent University, Ankara, Turkey.
Allergy ; 77(1): 258-270, 2022 01.
Article in English | MEDLINE | ID: covidwho-1406541
ABSTRACT

BACKGROUND:

Vaccines that incorporate multiple SARS-CoV-2 antigens can further broaden the breadth of virus-specific cellular and humoral immunity. This study describes the development and immunogenicity of SARS-CoV-2 VLP vaccine that incorporates the four structural proteins of SARS-CoV-2.

METHODS:

VLPs were generated in transiently transfected HEK293 cells, purified by multimodal chromatography, and characterized by tunable-resistive pulse sensing, AFM, SEM, and TEM. Immunoblotting studies verified the protein identities of VLPs. Cellular and humoral immune responses of immunized animals demonstrated the immune potency of the formulated VLP vaccine.

RESULTS:

Transiently transfected HEK293 cells reproducibly generated vesicular VLPs that were similar in size to and expressing all four structural proteins of SARS-CoV-2. Alum adsorbed, K3-CpG ODN-adjuvanted VLPs elicited high titer anti-S, anti-RBD, anti-N IgG, triggered multifunctional Th1-biased T-cell responses, reduced virus load, and prevented lung pathology upon live virus challenge in vaccinated animals.

CONCLUSION:

These data suggest that VLPs expressing all four structural protein antigens of SARS-CoV-2 are immunogenic and can protect animals from developing COVID-19 infection following vaccination.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Vaccines, Virus-Like Particle / COVID-19 Type of study: Experimental Studies Topics: Vaccines Limits: Animals / Humans Language: English Journal: Allergy Year: 2022 Document Type: Article Affiliation country: All.15091

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Vaccines, Virus-Like Particle / COVID-19 Type of study: Experimental Studies Topics: Vaccines Limits: Animals / Humans Language: English Journal: Allergy Year: 2022 Document Type: Article Affiliation country: All.15091