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Mix-and-read, one-minute SARS-CoV-2 diagnostic assay: development of PIFE-based aptasensor.
Lee, J Michelle; Kim, Chae Rin; Kim, Sion; Min, Junhong; Lee, Min-Ho; Lee, SangWook.
  • Lee JM; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
  • Kim CR; PCL, Inc., Rm 701, Star Valley, 99, Digital-ro-9-gil, Ge-umcheon-gu, Seoul, 08510, Republic of Korea. swlee@pclchip.com.
  • Kim S; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
  • Min J; PCL, Inc., Rm 701, Star Valley, 99, Digital-ro-9-gil, Ge-umcheon-gu, Seoul, 08510, Republic of Korea. swlee@pclchip.com.
  • Lee MH; PCL, Inc., Rm 701, Star Valley, 99, Digital-ro-9-gil, Ge-umcheon-gu, Seoul, 08510, Republic of Korea. swlee@pclchip.com.
  • Lee S; College of LSA, University of Michigan, Ann Arbor, MI, 48104, USA.
Chem Commun (Camb) ; 57(79): 10222-10225, 2021 Oct 05.
Article in English | MEDLINE | ID: covidwho-1408635
ABSTRACT
We developed a one-minute, one-step SARS-CoV-2 antigen assay based on protein-induced fluorescence enhancement of a DNA aptamer. The system showed significant selectivity and sensitivity towards both nucleocapsid protein and SARS-CoV-2 virus lysate, but with marked improvements in speed and manufacturability. We hence propose this platform as a mix-and-read testing strategy for SARS-CoV-2 that can be applied to POC diagnostics in clinical settings, especially in low- and middle-income countries.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Aptamers, Nucleotide / Coronavirus Nucleocapsid Proteins / COVID-19 Testing / SARS-CoV-2 / COVID-19 / Antigens, Viral Type of study: Diagnostic study / Prognostic study Language: English Journal: Chem Commun (Camb) Journal subject: Chemistry Year: 2021 Document Type: Article Affiliation country: D1cc04066a

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Aptamers, Nucleotide / Coronavirus Nucleocapsid Proteins / COVID-19 Testing / SARS-CoV-2 / COVID-19 / Antigens, Viral Type of study: Diagnostic study / Prognostic study Language: English Journal: Chem Commun (Camb) Journal subject: Chemistry Year: 2021 Document Type: Article Affiliation country: D1cc04066a