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Applications of digital PCR in COVID-19 pandemic.
Tan, Chianru; Fan, Dongdong; Wang, Nan; Wang, Fang; Wang, Bo; Zhu, Lingxiang; Guo, Yong.
  • Tan C; Department of Biomedical Engineering, School of Medicine Tsinghua University Beijing China.
  • Fan D; Department of Biomedical Engineering, School of Medicine Tsinghua University Beijing China.
  • Wang N; Department of Biomedical Engineering, School of Medicine Tsinghua University Beijing China.
  • Wang F; Department of Biomedical Engineering, School of Medicine Tsinghua University Beijing China.
  • Wang B; TargetingOne Corporation Beijing China.
  • Zhu L; National Research Institute for Family Planning Beijing China.
  • Guo Y; Department of Biomedical Engineering, School of Medicine Tsinghua University Beijing China.
View (Beijing) ; 2(2): 20200082, 2021 Apr.
Article in English | MEDLINE | ID: covidwho-1411072
ABSTRACT
The coronavirus disease 2019 (COVID-19) pandemic has led to a public health crisis and global panic. This infectious disease is caused by a novel coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Digital polymerase chain reaction (dPCR), which is an emerging nucleic acid amplification technology that allows absolute quantification of nucleic acids, plays an important role in the detection of SARS-CoV-2. In this review, we introduce the principle and advantages of dPCR, and review the applications of dPCR in the COVID-19 pandemic, including detection of low copy number viruses, measurement of the viral load, preparation of reference materials, monitoring of virus concentration in the environment, detection of viral mutations, and evaluation of anti-SARS-CoV-2 drugs. We also discuss the challenges of dPCR in clinical practice.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Prognostic study Language: English Journal: View (Beijing) Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Prognostic study Language: English Journal: View (Beijing) Year: 2021 Document Type: Article