Development and application of a SARS-CoV-2 colorimetric biosensor based on the peroxidase-mimic activity of γ-Fe2O3 nanoparticles.
Mikrochim Acta
; 188(10): 335, 2021 Sep 09.
Article
in English
| MEDLINE | ID: covidwho-1411927
ABSTRACT
A practical colorimetric assay was developed for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For this purpose, magnetic γ Fe2O3 nanoparticles were synthesized and used as a peroxidase-like mimic activity molecule. In the presence of γ Fe2O3 nanoparticles, the color change of H2O2 included 3,3',5,5'-tetramethylbenzidine was monitored at the wavelength of 654 nm when spike protein interacted with angiotensin-converting enzyme 2 receptor. This oxidation-reduction reaction was examined both spectroscopically and by using electrochemical techniques. The experimental parameters were optimized and the analytical characteristics investigated. The developed assay was applied to real SARS-CoV-2 samples, and very good results that were in accordance with the real time polymerase chain reaction were obtained.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Colorimetry
/
Spike Glycoprotein, Coronavirus
/
Magnetic Iron Oxide Nanoparticles
/
COVID-19 Testing
/
SARS-CoV-2
/
COVID-19
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Mikrochim Acta
Year:
2021
Document Type:
Article
Affiliation country:
S00604-021-04989-6
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