Bovine coronavirus in neonatal calf diarrhoea in Iran.
Vet Med Sci
; 6(4): 686-694, 2020 11.
Article
in English
| MEDLINE | ID: covidwho-141637
ABSTRACT
Partial gene sequencing for the bovine coronavirus at the World Genebank is available for many countries, which are distributed unevenly in five continents, but so far, no sequencing of strains has been recorded in Iran. One hundred ninety-four stool samples from calves with diarrhoea less than one-month old were collected from five different geographical regions of country in order to detect coronavirus and characterize it if coronavirus was found. Samples were screened for the presence of BCoV by using a commercially available ELISA kit. Furthermore, RT-PCR was carried out on positive samples for confirmation of the presence of N and S specific genes. Sequencing and phylogenetic analysis was carried out following RT-PCR tests. 7.2% of samples, were positive for BCoV and all stool samples from the South-West, Northeast and West regions of Iran were negative. The results showed that all the strains of coronavirus identified in Iran were completely in independent clusters and that they did not stand in the same cluster as any of the strains identified in other parts of the world. The strains from Iran were quite different from strains in other parts of the world but from the point of similarity these viruses showed some similarities to the European strains, such as those found in France, Croatia, Denmark and Sweden.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Cattle Diseases
/
Coronavirus, Bovine
/
Coronavirus Infections
/
Diarrhea
/
Animals, Newborn
Type of study:
Observational study
Limits:
Animals
Country/Region as subject:
Asia
Language:
English
Journal:
Vet Med Sci
Year:
2020
Document Type:
Article
Affiliation country:
Vms3.277
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