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Magnetofluidic immuno-PCR for point-of-care COVID-19 serological testing.
Zhang, Pengfei; Chen, Liben; Hu, Jiumei; Trick, Alexander Y; Chen, Fan-En; Hsieh, Kuangwen; Zhao, Yang; Coleman, Branch; Kruczynski, Kate; Pisanic, Thomas R; Heaney, Christopher D; Clarke, William A; Wang, Tza-Huei.
  • Zhang P; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
  • Chen L; Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
  • Hu J; Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
  • Trick AY; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
  • Chen FE; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
  • Hsieh K; Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
  • Zhao Y; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
  • Coleman B; Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.
  • Kruczynski K; Department of Environmental Health and Engineering, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, 21205, USA.
  • Pisanic TR; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, 21218, USA.
  • Heaney CD; Department of Environmental Health and Engineering, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, 21205, USA.
  • Clarke WA; Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.
  • Wang TH; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA; Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, 21218, USA. Electronic address: thwang@jhu.e
Biosens Bioelectron ; 195: 113656, 2022 Jan 01.
Article in English | MEDLINE | ID: covidwho-1432990
ABSTRACT
Serological tests play an important role in the fight against Coronavirus Disease 2019 (COVID-19), including monitoring the dynamic immune response after vaccination, identifying past infection and determining community infection rate. Conventional methods for serological testing, such as enzyme-linked immunosorbent assays and chemiluminescence immunoassays, provide reliable and sensitive antibody detection but require sophisticated laboratory infrastructure and/or lengthy assay time. Conversely, lateral flow immunoassays are suitable for rapid point-of-care tests but have limited sensitivity. Here, we describe the development of a rapid and sensitive magnetofluidic immuno-PCR platform that can address the current gap in point-of-care serological testing for COVID-19. Our magnetofluidic immuno-PCR platform automates a magnetic bead-based, single-binding, and one-wash immuno-PCR assay in a palm-sized magnetofluidic device and delivers results in ∼30 min. In the device, a programmable magnetic arm attracts and transports magnetically-captured antibodies through assay reagents pre-loaded in a companion plastic cartridge, and a miniaturized thermocycler and a fluorescence detector perform immuno-PCR to detect the antibodies. We evaluated our magnetofluidic immuno-PCR with 108 clinical serum/plasma samples and achieved 93.8% (45/48) sensitivity and 98.3% (59/60) specificity, demonstrating its potential as a rapid and sensitive point-of-care serological test for COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2022 Document Type: Article Affiliation country: J.bios.2021.113656

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2022 Document Type: Article Affiliation country: J.bios.2021.113656