Your browser doesn't support javascript.
Severity of COVID-19 Patients Predicted by Serum Sphingolipids Signature.
Torretta, Enrica; Garziano, Micaela; Poliseno, Mariacristina; Capitanio, Daniele; Biasin, Mara; Santantonio, Teresa Antonia; Clerici, Mario; Lo Caputo, Sergio; Trabattoni, Daria; Gelfi, Cecilia.
  • Torretta E; IRCCS Istituto Ortopedico Galeazzi, 20161 Milan, Italy.
  • Garziano M; Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti, Università degli Studi di Milano, 20122 Milan, Italy.
  • Poliseno M; Dipartimento di Scienze Biomediche e Cliniche "L. Sacco", Università degli Studi di Milano, 20157 Milan, Italy.
  • Capitanio D; Unit of Infectious Diseases, Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, Italy.
  • Biasin M; Department of Biomedical Sciences for Health, University of Milan, 20090 Segrate, Italy.
  • Santantonio TA; Dipartimento di Scienze Biomediche e Cliniche "L. Sacco", Università degli Studi di Milano, 20157 Milan, Italy.
  • Clerici M; Unit of Infectious Diseases, Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, Italy.
  • Lo Caputo S; Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti, Università degli Studi di Milano, 20122 Milan, Italy.
  • Trabattoni D; Don C. Gnocchi Foundation, IRCCS, 20148 Milan, Italy.
  • Gelfi C; Unit of Infectious Diseases, Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, Italy.
Int J Mol Sci ; 22(19)2021 Sep 22.
Article in English | MEDLINE | ID: covidwho-1438628
ABSTRACT
The reason behind the high inter-individual variability in response to SARS-CoV-2 infection and patient's outcome is poorly understood. The present study targets the sphingolipid profile of twenty-four healthy controls and fifty-nine COVID-19 patients with different disease severity. Sera were analyzed by untargeted and targeted mass spectrometry and ELISA. Results indicated a progressive increase in dihydrosphingosine, dihydroceramides, ceramides, sphingosine, and a decrease in sphingosine-1-phosphate. These changes are associated with a serine palmitoyltransferase long chain base subunit 1 (SPTLC1) increase in relation to COVID-19 severity. Severe patients showed a decrease in sphingomyelins and a high level of acid sphingomyelinase (aSMase) that influences monosialodihexosyl ganglioside (GM3) C160 levels. Critical patients are characterized by high levels of dihydrosphingosine and dihydroceramide but not of glycosphingolipids. In severe and critical patients, unbalanced lipid metabolism induces lipid raft remodeling, leads to cell apoptosis and immunoescape, suggesting active sphingolipid participation in viral infection. Furthermore, results indicated that the sphingolipid and glycosphingolipid metabolic rewiring promoted by aSMase and GM3 is age-dependent but also characteristic of severe and critical patients influencing prognosis and increasing viral load. AUCs calculated from ROC curves indicated ceramides C160, C180, C241, sphingosine and SPTLC1 as putative biomarkers of disease evolution.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Sphingolipids / COVID-19 Subject: Sphingolipids / COVID-19 Type of study: Diagnostic study / Prognostic study / Risk factors Language: English Clinical aspect: Prediction / Prognosis Year: 2021

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Sphingolipids / COVID-19 Subject: Sphingolipids / COVID-19 Type of study: Diagnostic study / Prognostic study / Risk factors Language: English Clinical aspect: Prediction / Prognosis Year: 2021
...