The intraviral protein-protein interaction of SARS-CoV-2 reveals the key role of N protein in virus-like particle assembly.
Int J Biol Sci
; 17(14): 3889-3897, 2021.
Article
in English
| MEDLINE | ID: covidwho-1438863
ABSTRACT
Intraviral protein-protein interactions (PPIs) of SARS-CoV-2 in host cells may provide useful information for deep understanding of virology of SARS-CoV-2. In this study, 22 of 55 interactions of the structural and accessory proteins of SARS-CoV-2 were identified by biomolecular fluorescence complementation (BiFC) assay. The nucleocapsid (N) protein was found to have the most interactions among the structural and accessory proteins of SARS-CoV-2, and also specifically interacted with the putative packaging signal (PS) of SARS-CoV-2. We also demonstrated that the PS core containing PS576 RNA bears a functional PS, important for the assembly of the viral RNA into virus like particles (VLPs), and the packaging of SARS-CoV-2 RNA was N dependent.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Virus Assembly
/
Coronavirus Nucleocapsid Proteins
/
SARS-CoV-2
Limits:
Humans
Language:
English
Journal:
Int J Biol Sci
Journal subject:
Biology
Year:
2021
Document Type:
Article
Affiliation country:
Ijbs.64977
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