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Ultrasensitive Detection of SARS-CoV-2 Antibody by Graphene Field-Effect Transistors.
Kang, Hua; Wang, Xuejun; Guo, Mingquan; Dai, Changhao; Chen, Renzhong; Yang, Lei; Wu, Yanling; Ying, Tianlei; Zhu, Zhaoqin; Wei, Dapeng; Liu, Yunqi; Wei, Dacheng.
  • Kang H; State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
  • Wang X; Institute of Molecular Materials and Devices, Department of Material Science, Fudan University, Shanghai 200433, China.
  • Guo M; State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
  • Dai C; Institute of Molecular Materials and Devices, Department of Material Science, Fudan University, Shanghai 200433, China.
  • Chen R; Department of Laboratory Medicine, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
  • Yang L; State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
  • Wu Y; Institute of Molecular Materials and Devices, Department of Material Science, Fudan University, Shanghai 200433, China.
  • Ying T; State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
  • Zhu Z; Institute of Molecular Materials and Devices, Department of Material Science, Fudan University, Shanghai 200433, China.
  • Wei D; State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
  • Liu Y; Institute of Molecular Materials and Devices, Department of Material Science, Fudan University, Shanghai 200433, China.
  • Wei D; MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
Nano Lett ; 21(19): 7897-7904, 2021 10 13.
Article in English | MEDLINE | ID: covidwho-1440453
ABSTRACT
The fast spread of SARS-CoV-2 has severely threatened the public health. Establishing a sensitive method for SARS-CoV-2 detection is of great significance to contain the worldwide pandemic. Here, we develop a graphene field-effect transistor (g-FET) biosensor and realize ultrasensitive SARS-CoV-2 antibody detection with a limit of detection (LoD) down to 10-18 M (equivalent to 10-16 g mL-1) level. The g-FETs are modified with spike S1 proteins, and the SARS-CoV-2 antibody biorecognition events occur in the vicinity of the graphene surface, yielding an LoD of ∼150 antibodies in 100 µL full serum, which is the lowest LoD value of antibody detection. The diagnoses time is down to 2 min for detecting clinical serum samples. As such, the g-FETs leverage rapid and precise SARS-CoV-2 screening and also hold great promise in prevention and control of other epidemic outbreaks in the future.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 / Graphite Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: Nano Lett Year: 2021 Document Type: Article Affiliation country: Acs.nanolett.1c00837

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 / Graphite Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: Nano Lett Year: 2021 Document Type: Article Affiliation country: Acs.nanolett.1c00837