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1H, 13C, and 15N backbone chemical-shift assignments of SARS-CoV-2 non-structural protein 1 (leader protein).
Wang, Ying; Kirkpatrick, John; Zur Lage, Susanne; Korn, Sophie M; Neißner, Konstantin; Schwalbe, Harald; Schlundt, Andreas; Carlomagno, Teresa.
  • Wang Y; Centre of Biomolecular Drug Research (BMWZ), Leibniz University Hannover, Schneiderberg 38, 30167, Hannover, Germany.
  • Kirkpatrick J; Centre of Biomolecular Drug Research (BMWZ), Leibniz University Hannover, Schneiderberg 38, 30167, Hannover, Germany.
  • Zur Lage S; Group of NMR-Based Structural Chemistry, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124, Braunschweig, Germany.
  • Korn SM; Group of NMR-Based Structural Chemistry, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124, Braunschweig, Germany.
  • Neißner K; Institute for Molecular Biosciences, St Lucia, QLD, 4072, Australia.
  • Schwalbe H; Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt, Germany.
  • Schlundt A; Institute for Molecular Biosciences, St Lucia, QLD, 4072, Australia.
  • Carlomagno T; Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt, Germany.
Biomol NMR Assign ; 15(2): 287-295, 2021 10.
Article in English | MEDLINE | ID: covidwho-1442183
Semantic information from SemMedBD (by NLM)
1. New medications TREATS Disease
Subject
New medications
Predicate
TREATS
Object
Disease
2. Polyproteins PART_OF replicase
Subject
Polyproteins
Predicate
PART_OF
Object
replicase
3. Polyproteins PRODUCES Structural protein
Subject
Polyproteins
Predicate
PRODUCES
Object
Structural protein
4. 5\' Untranslated Regions INTERACTS_WITH RN
Subject
5\' Untranslated Regions
Predicate
INTERACTS_WITH
Object
RN
5. Evaluation procedure USES Magnetic Resonance Imaging
Subject
Evaluation procedure
Predicate
USES
Object
Magnetic Resonance Imaging
6. New medications TREATS Disease
Subject
New medications
Predicate
TREATS
Object
Disease
7. Polyproteins PART_OF replicase
Subject
Polyproteins
Predicate
PART_OF
Object
replicase
8. Polyproteins PRODUCES Structural protein
Subject
Polyproteins
Predicate
PRODUCES
Object
Structural protein
9. 5' Untranslated Regions INTERACTS_WITH RNA, Viral
Subject
5' Untranslated Regions
Predicate
INTERACTS_WITH
Object
RNA, Viral
10. Evaluation procedure USES Magnetic Resonance Imaging
Subject
Evaluation procedure
Predicate
USES
Object
Magnetic Resonance Imaging
ABSTRACT
The current COVID-19 pandemic caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has become a worldwide health crisis, necessitating coordinated scientific research and urgent identification of new drug targets for treatment of COVID-19 lung disease. The covid19-nmr consortium seeks to support drug development by providing publicly accessible NMR data on the viral RNA elements and proteins. The SARS-CoV-2 genome comprises a single RNA of about 30 kb in length, in which 14 open reading frames (ORFs) have been annotated, and encodes approximately 30 proteins. The first two-thirds of the SARS-CoV-2 genome is made up of two large overlapping open-reading-frames (ORF1a and ORF1b) encoding a replicase polyprotein, which is subsequently cleaved to yield 16 so-called non-structural proteins. The non-structural protein 1 (Nsp1), which is considered to be a major virulence factor, suppresses host immune functions by associating with host ribosomal complexes at the very end of its C-terminus. Furthermore, Nsp1 facilitates initiation of viral RNA translation via an interaction of its N-terminal domain with the 5' untranslated region (UTR) of the viral RNA. Here, we report the near-complete backbone chemical-shift assignments of full-length SARS-CoV-2 Nsp1 (19.8 kDa), which reveal the domain organization, secondary structure and backbone dynamics of Nsp1, and which will be of value to further NMR-based investigations of both the biochemical and physiological functions of Nsp1.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Nonstructural Proteins / Nuclear Magnetic Resonance, Biomolecular / SARS-CoV-2 Type of study: Prognostic study Language: English Journal: Biomol NMR Assign Journal subject: Molecular Biology / Nuclear Medicine Year: 2021 Document Type: Article Affiliation country: S12104-021-10019-6

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Nonstructural Proteins / Nuclear Magnetic Resonance, Biomolecular / SARS-CoV-2 Type of study: Prognostic study Language: English Journal: Biomol NMR Assign Journal subject: Molecular Biology / Nuclear Medicine Year: 2021 Document Type: Article Affiliation country: S12104-021-10019-6