Your browser doesn't support javascript.
1H, 13C, 15N and 31P chemical shift assignment for stem-loop 4 from the 5'-UTR of SARS-CoV-2.
Vögele, Jennifer; Ferner, Jan-Peter; Altincekic, Nadide; Bains, Jasleen Kaur; Ceylan, Betül; Fürtig, Boris; Grün, J Tassilo; Hengesbach, Martin; Hohmann, Katharina F; Hymon, Daniel; Knezic, Bozana; Löhr, Frank; Peter, Stephen A; Pyper, Dennis; Qureshi, Nusrat S; Richter, Christian; Schlundt, Andreas; Schwalbe, Harald; Stirnal, Elke; Sudakov, Alexey; Wacker, Anna; Weigand, Julia E; Wirmer-Bartoschek, Julia; Wöhnert, Jens; Duchardt-Ferner, Elke.
  • Vögele J; Institute for Molecular Biosciences, Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt/M., Germany.
  • Ferner JP; Center for Biomolecular Magnetic Resonance (BMRZ), Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Altincekic N; Center for Biomolecular Magnetic Resonance (BMRZ), Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Bains JK; Institute for Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Ceylan B; Center for Biomolecular Magnetic Resonance (BMRZ), Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Fürtig B; Institute for Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Grün JT; Center for Biomolecular Magnetic Resonance (BMRZ), Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Hengesbach M; Institute for Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Hohmann KF; Center for Biomolecular Magnetic Resonance (BMRZ), Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Hymon D; Institute for Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Knezic B; Center for Biomolecular Magnetic Resonance (BMRZ), Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Löhr F; Institute for Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Peter SA; Center for Biomolecular Magnetic Resonance (BMRZ), Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Pyper D; Institute for Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Qureshi NS; Center for Biomolecular Magnetic Resonance (BMRZ), Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Richter C; Institute for Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Schlundt A; Center for Biomolecular Magnetic Resonance (BMRZ), Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Schwalbe H; Institute for Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Stirnal E; Center for Biomolecular Magnetic Resonance (BMRZ), Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Sudakov A; Institute for Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Wacker A; Center for Biomolecular Magnetic Resonance (BMRZ), Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Weigand JE; Institute for Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Wirmer-Bartoschek J; Center for Biomolecular Magnetic Resonance (BMRZ), Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Wöhnert J; Institute for Biophysical Chemistry, Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt/M., Germany.
  • Duchardt-Ferner E; Department of Biology, Technical University of Darmstadt, Schnittspahnstr. 10, 64287, Darmstadt, Germany.
Biomol NMR Assign ; 15(2): 335-340, 2021 10.
Article in English | MEDLINE | ID: covidwho-1442184
Semantic information from SemMedBD (by NLM)
1. 5\' Untranslated Regions PART_OF 2019 novel coronavirus
Subject
5\' Untranslated Regions
Predicate
PART_OF
Object
2019 novel coronavirus
2. 2019 novel coronavirus CAUSES COVID-19
Subject
2019 novel coronavirus
Predicate
CAUSES
Object
COVID-19
3. Therapeutic procedure TREATS Disease
Subject
Therapeutic procedure
Predicate
TREATS
Object
Disease
4. Antiviral Agents COEXISTS_WITH Viral Genome
Subject
Antiviral Agents
Predicate
COEXISTS_WITH
Object
Viral Genome
5. 5\' Untranslated Regions AFFECTS Virus Replication
Subject
5\' Untranslated Regions
Predicate
AFFECTS
Object
Virus Replication
6. 5' Untranslated Regions PART_OF 2019 novel coronavirus
Subject
5' Untranslated Regions
Predicate
PART_OF
Object
2019 novel coronavirus
7. 2019 novel coronavirus CAUSES COVID-19
Subject
2019 novel coronavirus
Predicate
CAUSES
Object
COVID-19
8. Therapeutic procedure TREATS Disease
Subject
Therapeutic procedure
Predicate
TREATS
Object
Disease
9. Antiviral Agents COEXISTS_WITH Viral Genome
Subject
Antiviral Agents
Predicate
COEXISTS_WITH
Object
Viral Genome
10. 5' Untranslated Regions AFFECTS Virus Replication
Subject
5' Untranslated Regions
Predicate
AFFECTS
Object
Virus Replication
ABSTRACT
The SARS-CoV-2 virus is the cause of the respiratory disease COVID-19. As of today, therapeutic interventions in severe COVID-19 cases are still not available as no effective therapeutics have been developed so far. Despite the ongoing development of a number of effective vaccines, therapeutics to fight the disease once it has been contracted will still be required. Promising targets for the development of antiviral agents against SARS-CoV-2 can be found in the viral RNA genome. The 5'- and 3'-genomic ends of the 30 kb SCoV-2 genome are highly conserved among Betacoronaviruses and contain structured RNA elements involved in the translation and replication of the viral genome. The 40 nucleotides (nt) long highly conserved stem-loop 4 (5_SL4) is located within the 5'-untranslated region (5'-UTR) important for viral replication. 5_SL4 features an extended stem structure disrupted by several pyrimidine mismatches and is capped by a pentaloop. Here, we report extensive 1H, 13C, 15N and 31P resonance assignments of 5_SL4 as the basis for in-depth structural and ligand screening studies by solution NMR spectroscopy.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Nuclear Magnetic Resonance, Biomolecular / 5' Untranslated Regions / SARS-CoV-2 Topics: Vaccines Language: English Journal: Biomol NMR Assign Journal subject: Molecular Biology / Nuclear Medicine Year: 2021 Document Type: Article Affiliation country: S12104-021-10026-7

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Nuclear Magnetic Resonance, Biomolecular / 5' Untranslated Regions / SARS-CoV-2 Topics: Vaccines Language: English Journal: Biomol NMR Assign Journal subject: Molecular Biology / Nuclear Medicine Year: 2021 Document Type: Article Affiliation country: S12104-021-10026-7