Your browser doesn't support javascript.
Inhibitory Effects and Surface Plasmon Resonance-Based Binding Affinities of Dietary Hydrolyzable Tannins and Their Gut Microbial Metabolites on SARS-CoV-2 Main Protease.
Li, Huifang; Xu, Feng; Liu, Chang; Cai, Ang; Dain, Joel A; Li, Dongli; Seeram, Navindra P; Cho, Bongsup P; Ma, Hang.
  • Li H; School of Biotechnology and Health Sciences, Wuyi University, International Healthcare Innovation Institute (Jiangmen), Jiangmen 529020, China.
  • Xu F; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, Rhode Island 02881, United States.
  • Liu C; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, Rhode Island 02881, United States.
  • Cai A; College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550001, China.
  • Dain JA; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, Rhode Island 02881, United States.
  • Li D; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, Rhode Island 02881, United States.
  • Seeram NP; Department of Chemistry, University of Rhode Island, Kingston, Rhode Island 02881, United States.
  • Cho BP; Department of Chemistry, University of Rhode Island, Kingston, Rhode Island 02881, United States.
  • Ma H; School of Biotechnology and Health Sciences, Wuyi University, International Healthcare Innovation Institute (Jiangmen), Jiangmen 529020, China.
J Agric Food Chem ; 69(41): 12197-12208, 2021 Oct 20.
Article in English | MEDLINE | ID: covidwho-1442683
ABSTRACT
Severe acute respiratory syndrome coronavirus (SARS-CoV-2) main protease (Mpro) inhibitors are considered as potential treatments for coronavirus disease 2019, and dietary polyphenols show promise in SARS-CoV-2 Mpro inhibition based on in silico studies. In the present study, we utilize a combination of biochemical-, surface plasmon resonance-, and docking-based assays to evaluate the inhibition and binding affinities of a series of tannins and their gut microbial metabolites on SARS-CoV-2 Mpro. The tested compounds (2-50 µM) were hydrolyzable tannins, including ellagitannins (punicalagin and ellagic acid) and gallotannins (tannic acid, pentagalloyl glucose, ginnalin A, and gallic acid), and their gut microbial metabolites, urolithins and pyrogallol, respectively. They inhibited SARS-CoV-2 Mpro (by 6.6-100.0% at 50 µM) and bound directly to the Mpro protein (with dissociation constants from 1.1 × 10-6 to 5.3 × 10-5 M). This study sheds light on the inhibitory effects of tannins and their metabolites on SARS-CoV-2 Mpro.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Gastrointestinal Microbiome / COVID-19 Limits: Humans Language: English Journal: J Agric Food Chem Year: 2021 Document Type: Article Affiliation country: Acs.jafc.1c03521

Full text: Available Collection: International databases Database: MEDLINE Main subject: Gastrointestinal Microbiome / COVID-19 Limits: Humans Language: English Journal: J Agric Food Chem Year: 2021 Document Type: Article Affiliation country: Acs.jafc.1c03521