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SARS-CoV-2 triggers DNA damage response in Vero E6 cells.
Victor, Joshua; Deutsch, Jamie; Whitaker, Annalis; Lamkin, Erica N; March, Anthony; Zhou, Pei; Botten, Jason W; Chatterjee, Nimrat.
  • Victor J; Department of Microbiology and Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA.
  • Deutsch J; Department of Microbiology and Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA.
  • Whitaker A; Division of Immunobiology, Department of Medicine, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA.
  • Lamkin EN; Department of Microbiology and Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA.
  • March A; Department of Microbiology and Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA.
  • Zhou P; Department of Biochemistry, Duke University School of Medicine, Durham, NC, 27710, USA.
  • Botten JW; Department of Microbiology and Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA; Division of Immunobiology, Department of Medicine, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA.
  • Chatterjee N; Department of Microbiology and Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA; Division of Immunobiology, Department of Medicine, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA; University of Vermont Cancer Center, Uni
Biochem Biophys Res Commun ; 579: 141-145, 2021 11 19.
Article in English | MEDLINE | ID: covidwho-1446456
Preprint
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ABSTRACT
The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus responsible for the current COVID-19 pandemic and has now infected more than 200 million people with more than 4 million deaths globally. Recent data suggest that symptoms and general malaise may continue long after the infection has ended in recovered patients, suggesting that SARS-CoV-2 infection has profound consequences in the host cells. Here we report that SARS-CoV-2 infection can trigger a DNA damage response (DDR) in African green monkey kidney cells (Vero E6). We observed a transcriptional upregulation of the Ataxia telangiectasia and Rad3 related protein (ATR) in infected cells. In addition, we observed enhanced phosphorylation of CHK1, a downstream effector of the ATR DNA damage response, as well as H2AX. Strikingly, SARS-CoV-2 infection lowered the expression of TRF2 shelterin-protein complex, and reduced telomere lengths in infected Vero E6 cells. Thus, our observations suggest SARS-CoV-2 may have pathological consequences to host cells beyond evoking an immunopathogenic immune response.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: DNA Damage / Host-Pathogen Interactions / SARS-CoV-2 / COVID-19 Type of study: Observational study / Prognostic study Topics: Long Covid Limits: Animals Language: English Journal: Biochem Biophys Res Commun Year: 2021 Document Type: Article Affiliation country: J.bbrc.2021.09.024

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Full text: Available Collection: International databases Database: MEDLINE Main subject: DNA Damage / Host-Pathogen Interactions / SARS-CoV-2 / COVID-19 Type of study: Observational study / Prognostic study Topics: Long Covid Limits: Animals Language: English Journal: Biochem Biophys Res Commun Year: 2021 Document Type: Article Affiliation country: J.bbrc.2021.09.024