Intrathecal inflammatory responses in the absence of SARS-CoV-2 nucleic acid in the CSF of COVID-19 hospitalized patients
Neurology
; 98(18 SUPPL), 2022.
Article
in English
| EMBASE | ID: covidwho-1925419
ABSTRACT
Objective:
Evaluate SARS-CoV-2 RNA and inflammatory cytokines and chemokines in the CSF of patients with acute COVID-19 and neurologic symptoms, and to compare these to controls and patients with known neurotropic pathogens.Background:
Neurologic symptoms have been described in 30-60% of hospitalized patients with Coronavirus Disease 2019 (COVID-19). However, little is known about CSF profiles in these patients. Design/Methods:
CSF from twenty-seven consecutive patients with COVID-19 and neurological symptoms was assayed for SARS-CoV-2 RNA using quantitative reverse transcription PCR (RT-qPCR) and unbiased metagenomic sequencing. Assays for blood brain barrier (BBB) breakdown (CSFserum albumin ratio (Q-Alb)), and proinflammatory cytokines and chemokines (IL-6, IL-8, IL-15, IL-16, monocyte chemoattractant protein -1 (MCP-1) and monocyte inhibitory protein - 1β (MIP-1β)) were performed in 23 patients and compared to CSF from patients with HIV-1 (16 virally suppressed, 5 unsuppressed), West Nile virus (WNV) (n=4) and 16 healthy controls (HC).Results:
Median CSF cell count for COVID-19 patients was 1 white blood cell/μL;two patients were infected with a second pathogen (Neisseria, Cryptococcus neoformans). No CSF samples had detectable SARS-CoV-2 RNA by either detection method. In patients with COVID-19 only, CSF IL-6, IL-8, IL-15, and MIP-1β levels were higher than HC and suppressed HIV (corrected-p < 0.05). MCP-1 and MIP-1β levels were higher, while IL-6, IL-8, IL-15 were similar in COVID-19 compared to WNV patients. Q-Alb correlated with all proinflammatory markers, with IL-6, IL-8, and MIP-1β (r≥0.6, p<0.01) demonstrating the strongest associations.Conclusions:
Lack of SARS-CoV-2 RNA in CSF is consistent with pre-existing literature. Evidence of intrathecal proinflammatory markers in a subset of COVID-19 patients with BBB breakdown despite minimal CSF pleocytosis is atypical for neurotropic pathogens.
chemokine; cytokine; endogenous compound; interleukin 15; interleukin 16; interleukin 6; interleukin 8; macrophage inflammatory protein 1beta; monocyte chemotactic protein 1; serum albumin; adult; blood brain barrier; case report; cerebrospinal fluid; cerebrospinal fluid cytology; clinical article; conference abstract; coronavirus disease 2019; Cryptococcus neoformans; female; gene expression; hospital patient; human; human cell; Human immunodeficiency virus 1; infectious agent; inflammation; leukocyte; male; metagenomics; monocyte; Neisseria; neurologic disease; nonhuman; pleocytosis; quantitative analysis; real time reverse transcription polymerase chain reaction; Severe acute respiratory syndrome coronavirus 2; West Nile virus
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Collection:
Databases of international organizations
Database:
EMBASE
Language:
English
Journal:
Neurology
Year:
2022
Document Type:
Article
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