Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA.
Sci Rep
; 11(1): 19579, 2021 10 01.
Article
in English
| MEDLINE | ID: covidwho-1447327
ABSTRACT
The increasing risk from viral outbreaks such as the ongoing COVID-19 pandemic exacerbates the need for rapid, affordable and sensitive methods for virus detection, identification and quantification; however, existing methods for detecting virus particles in biological samples usually depend on multistep protocols that take considerable time to yield a result. Here, we introduce a rapid fluorescence in situ hybridization (FISH) protocol capable of detecting influenza virus, avian infectious bronchitis virus and SARS-CoV-2 specifically and quantitatively in approximately 20 min, in virus cultures, combined nasal and throat swabs with added virus and likely patient samples without previous purification. This fast and facile workflow can be adapted both as a lab technique and a future diagnostic tool in enveloped viruses with an accessible genome.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Viruses
/
RNA, Viral
/
In Situ Hybridization, Fluorescence
Type of study:
Diagnostic study
/
Prognostic study
Language:
English
Journal:
Sci Rep
Year:
2021
Document Type:
Article
Affiliation country:
S41598-021-98972-z
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