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Children Have Similar Reverse Transcription Polymerase Chain Reaction Cycle Threshold for Severe Acute Respiratory Syndrome Coronavirus 2 in Comparison With Adults.
Polese-Bonatto, Márcia; Sartor, Ivaine Tais Sauthier; Varela, Fernanda Hammes; Giannini, Gabriela Luchiari Tumioto; Azevedo, Thaís Raupp; Kern, Luciane Beatriz; Fernandes, Ingrid Rodrigues; Zavaglia, Gabriela Oliveira; de David, Caroline Nespolo; Santos, Amanda Paz; de Almeida, Walquiria Aparecida Ferreira; Porto, Victor Bertollo Gomes; Scotta, Marcelo Comerlato; Stein, Renato T.
  • Polese-Bonatto M; From the Social Responsibility - PROADI-SUS, Hospital Moinhos de Vento.
  • Sartor ITS; From the Social Responsibility - PROADI-SUS, Hospital Moinhos de Vento.
  • Varela FH; From the Social Responsibility - PROADI-SUS, Hospital Moinhos de Vento.
  • Giannini GLT; School of Medicine, Pontifícia Universidade Católica do Rio Grande do Sul.
  • Azevedo TR; Laboratory of Genetics and Molecular Biology, Hospital Moinhos de Vento, Porto Alegre, Brazil.
  • Kern LB; From the Social Responsibility - PROADI-SUS, Hospital Moinhos de Vento.
  • Fernandes IR; From the Social Responsibility - PROADI-SUS, Hospital Moinhos de Vento.
  • Zavaglia GO; From the Social Responsibility - PROADI-SUS, Hospital Moinhos de Vento.
  • de David CN; From the Social Responsibility - PROADI-SUS, Hospital Moinhos de Vento.
  • Santos AP; From the Social Responsibility - PROADI-SUS, Hospital Moinhos de Vento.
  • de Almeida WAF; From the Social Responsibility - PROADI-SUS, Hospital Moinhos de Vento.
  • Porto VBG; General Coordination, National Immunization Program, Brazilian Ministry of Health, Brasilia, Brazil.
  • Scotta MC; General Coordination, National Immunization Program, Brazilian Ministry of Health, Brasilia, Brazil.
  • Stein RT; From the Social Responsibility - PROADI-SUS, Hospital Moinhos de Vento.
Pediatr Infect Dis J ; 40(11): e413-e417, 2021 11 01.
Article in English | MEDLINE | ID: covidwho-1447654
ABSTRACT

BACKGROUND:

The viral dynamics and the role of children in the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are not completely understood. Our aim was to evaluate reverse transcription polymerase chain reaction (RT-PCR) cycle threshold (Ct) values among children with confirmed SARS-CoV-2 compared with that of adult subjects.

METHODS:

Patients (from 2 months to ≤18 years of age and adults) with signs and symptoms of acute SARS-CoV-2 infection for less than 7 days were prospectively enrolled in the study from May to November 2020. All participants performed RT-PCR assay for SARS-CoV-2 detection; Ct values of ORF1ab, N and S gene targets and the average of all the 3 probes were used as surrogates of viral load.

RESULTS:

There were 21 infants (2 months to <2 years), 40 children (≥2 to <12 years), 22 adolescents (≥12 to <18 years) and 293 adults of 376 participants with confirmed SARS-CoV-2 infections. RT-PCR Ct values from all participants less than 18 years of age, as well as from all childhood subgroups, were not significantly different from adults, comparing ORF1ab, N, S and all the gene targets together (P = 0.453).

CONCLUSIONS:

Ct values for children were comparable with that of adults. Although viral load is not the only determinant of SARS-CoV-2 transmission, children may play a role in the spread of coronavirus disease 2019 in the community.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Reverse Transcriptase Polymerase Chain Reaction / COVID-19 Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Observational study / Randomized controlled trials Limits: Adolescent / Adult / Child / Humans / Infant Country/Region as subject: South America / Brazil Language: English Journal: Pediatr Infect Dis J Journal subject: Communicable Diseases / Pediatrics Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Reverse Transcriptase Polymerase Chain Reaction / COVID-19 Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Observational study / Randomized controlled trials Limits: Adolescent / Adult / Child / Humans / Infant Country/Region as subject: South America / Brazil Language: English Journal: Pediatr Infect Dis J Journal subject: Communicable Diseases / Pediatrics Year: 2021 Document Type: Article