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Intradermal-delivered DNA vaccine induces durable immunity mediating a reduction in viral load in a rhesus macaque SARS-CoV-2 challenge model.
Patel, Ami; Walters, Jewell N; Reuschel, Emma L; Schultheis, Katherine; Parzych, Elizabeth; Gary, Ebony N; Maricic, Igor; Purwar, Mansi; Eblimit, Zeena; Walker, Susanne N; Guimet, Diana; Bhojnagarwala, Pratik; Adeniji, Opeyemi S; Doan, Arthur; Xu, Ziyang; Elwood, Dustin; Reeder, Sophia M; Pessaint, Laurent; Kim, Kevin Y; Cook, Anthony; Chokkalingam, Neethu; Finneyfrock, Brad; Tello-Ruiz, Edgar; Dodson, Alan; Choi, Jihae; Generotti, Alison; Harrison, John; Tursi, Nicholas J; Andrade, Viviane M; Dia, Yaya; Zaidi, Faraz I; Andersen, Hanne; Abdel-Mohsen, Mohamed; Lewis, Mark G; Muthumani, Kar; Kim, J Joseph; Kulp, Daniel W; Humeau, Laurent M; Ramos, Stephanie J; Smith, Trevor R F; Weiner, David B; Broderick, Kate E.
  • Patel A; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Walters JN; Inovio Pharmaceuticals, Plymouth Meeting, PA 19462, USA.
  • Reuschel EL; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Schultheis K; Inovio Pharmaceuticals, Plymouth Meeting, PA 19462, USA.
  • Parzych E; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Gary EN; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Maricic I; Inovio Pharmaceuticals, Plymouth Meeting, PA 19462, USA.
  • Purwar M; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Eblimit Z; Inovio Pharmaceuticals, Plymouth Meeting, PA 19462, USA.
  • Walker SN; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Guimet D; Inovio Pharmaceuticals, Plymouth Meeting, PA 19462, USA.
  • Bhojnagarwala P; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Adeniji OS; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Doan A; Inovio Pharmaceuticals, Plymouth Meeting, PA 19462, USA.
  • Xu Z; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Elwood D; Inovio Pharmaceuticals, Plymouth Meeting, PA 19462, USA.
  • Reeder SM; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Pessaint L; Bioqual, Rockville, MD 20852, USA.
  • Kim KY; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Cook A; Bioqual, Rockville, MD 20852, USA.
  • Chokkalingam N; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Finneyfrock B; Bioqual, Rockville, MD 20852, USA.
  • Tello-Ruiz E; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Dodson A; Bioqual, Rockville, MD 20852, USA.
  • Choi J; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Generotti A; Inovio Pharmaceuticals, Plymouth Meeting, PA 19462, USA.
  • Harrison J; Bioqual, Rockville, MD 20852, USA.
  • Tursi NJ; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Andrade VM; Inovio Pharmaceuticals, Plymouth Meeting, PA 19462, USA.
  • Dia Y; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Zaidi FI; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Andersen H; Bioqual, Rockville, MD 20852, USA.
  • Abdel-Mohsen M; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Lewis MG; Bioqual, Rockville, MD 20852, USA.
  • Muthumani K; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Kim JJ; Inovio Pharmaceuticals, Plymouth Meeting, PA 19462, USA.
  • Kulp DW; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Humeau LM; Inovio Pharmaceuticals, Plymouth Meeting, PA 19462, USA.
  • Ramos SJ; Inovio Pharmaceuticals, Plymouth Meeting, PA 19462, USA.
  • Smith TRF; Inovio Pharmaceuticals, Plymouth Meeting, PA 19462, USA.
  • Weiner DB; Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA.
  • Broderick KE; Inovio Pharmaceuticals, Plymouth Meeting, PA 19462, USA.
Cell Rep Med ; 2(10): 100420, 2021 10 19.
Article in English | MEDLINE | ID: covidwho-1450242
ABSTRACT
Coronavirus disease 2019 (COVID-19), caused by the SARS-CoV-2 virus, has had a dramatic global impact on public health and social and economic infrastructures. Here, we assess the immunogenicity and anamnestic protective efficacy in rhesus macaques of an intradermal (i.d.)-delivered SARS-CoV-2 spike DNA vaccine, INO-4800, currently being evaluated in clinical trials. Vaccination with INO-4800 induced T cell responses and induced spike antigen and RBD binding antibodies with ADCP and ADCD activity. Sera from the animals neutralized both the D614 and G614 SARS-CoV-2 pseudotype viruses. Several months after vaccination, animals were challenged with SARS-CoV-2 resulting in rapid recall of anti-SARS-CoV-2 spike proteincell and neutralizing antibody responses. These responses were associated with lower viral loads in the lung. These studies support the immune impact of INO-4800 for inducing both humoral and cellular arms of the adaptive immune system, which are likely important for providing durable protection against COVID-19 disease.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: T-Lymphocytes / COVID-19 Vaccines / COVID-19 / Lung / Antibodies, Viral Type of study: Experimental Studies / Prognostic study Topics: Vaccines Limits: Animals Language: English Journal: Cell Rep Med Year: 2021 Document Type: Article Affiliation country: J.xcrm.2021.100420

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Full text: Available Collection: International databases Database: MEDLINE Main subject: T-Lymphocytes / COVID-19 Vaccines / COVID-19 / Lung / Antibodies, Viral Type of study: Experimental Studies / Prognostic study Topics: Vaccines Limits: Animals Language: English Journal: Cell Rep Med Year: 2021 Document Type: Article Affiliation country: J.xcrm.2021.100420