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Crystal structures of human coronavirus NL63 main protease at different pH values.
Gao, Hongxia; Zhang, Yuting; Jiang, Haihai; Hu, Xiaohui; Zhang, Yuting; Zhou, Xuelan; Zhong, Fanglin; Lin, Cheng; Li, Jian; Luo, Jun; Zhang, Jin.
  • Gao H; Department of Rehabilitation Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China.
  • Zhang Y; School of Basic Medical Sciences, Nanchang University, Nanchang, Jiangxi 330031, People's Republic of China.
  • Jiang H; School of Basic Medical Sciences, Nanchang University, Nanchang, Jiangxi 330031, People's Republic of China.
  • Hu X; School of Basic Medical Sciences, Nanchang University, Nanchang, Jiangxi 330031, People's Republic of China.
  • Zhang Y; School of Basic Medical Sciences, Nanchang University, Nanchang, Jiangxi 330031, People's Republic of China.
  • Zhou X; School of Basic Medical Sciences, Nanchang University, Nanchang, Jiangxi 330031, People's Republic of China.
  • Zhong F; Shenzhen Crystalo Biopharmaceutical Co. Ltd, Shenzhen, Guangdong 518118, People's Republic of China.
  • Lin C; Shenzhen Crystalo Biopharmaceutical Co. Ltd, Shenzhen, Guangdong 518118, People's Republic of China.
  • Li J; College of Pharmaceutical Sciences, Gannan Medical University, Ganzhou, Jiangxi 341000, People's Republic of China.
  • Luo J; Department of Rehabilitation Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China.
  • Zhang J; School of Basic Medical Sciences, Nanchang University, Nanchang, Jiangxi 330031, People's Republic of China.
Acta Crystallogr F Struct Biol Commun ; 77(Pt 10): 348-355, 2021 Oct 01.
Article in English | MEDLINE | ID: covidwho-1450488
ABSTRACT
Human coronavirus NL63 (HCoV-NL63), which belongs to the genus Alphacoronavirus, mainly infects children and the immunocompromized and is responsible for a series of clinical manifestations, including cough, fever, rhinorrhoea, bronchiolitis and croup. HCoV-NL63, which was first isolated from a seven-month-old child in 2004, has led to infections worldwide and accounts for 10% of all respiratory illnesses caused by etiological agents. However, effective antivirals against HCoV-NL63 infection are currently unavailable. The HCoV-NL63 main protease (Mpro), also called 3C-like protease (3CLpro), plays a vital role in mediating viral replication and transcription by catalyzing the cleavage of replicase polyproteins (pp1a and pp1ab) into functional subunits. Moreover, Mpro is highly conserved among all coronaviruses, thus making it a prominent drug target for antiviral therapy. Here, four crystal structures of HCoV-NL63 Mpro in the apo form at different pH values are reported at resolutions of up to 1.78 Å. Comparison with Mpro from other human betacoronaviruses such as SARS-CoV-2 and SARS-CoV reveals common and distinct structural features in different genera and extends knowledge of the diversity, function and evolution of coronaviruses.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Crystallography, X-Ray / Crystallization / Coronavirus NL63, Human Type of study: Etiology study / Prognostic study Limits: Humans Language: English Journal: Acta Crystallogr F Struct Biol Commun Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Crystallography, X-Ray / Crystallization / Coronavirus NL63, Human Type of study: Etiology study / Prognostic study Limits: Humans Language: English Journal: Acta Crystallogr F Struct Biol Commun Year: 2021 Document Type: Article