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Detection of SARS-CoV2 virus using the real-time reverse transcriptase polymerase chain reaction in semen and seminal plasma from men with active COVID-19 infection - A pilot study.
Gupta, Pankush; Choudhary, Aashish; Gopal, Giridara; Kumar, Rajeev; Kumar, Arbind; Tiwari, Pawan; Malhotra, Neena.
  • Gupta P; Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, New Delhi, India.
  • Choudhary A; Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India.
  • Gopal G; Department of Community Medicine, All India Institute of Medical Sciences, New Delhi, India.
  • Kumar R; Department of Urology, All India Institute of Medical Sciences, New Delhi, India.
  • Kumar A; Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India.
  • Tiwari P; Department of Pulmonary Medicine, All India Institute of Medical Sciences, New Delhi, India.
  • Malhotra N; Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, New Delhi, India.
Indian J Urol ; 37(4): 331-334, 2021.
Article in English | MEDLINE | ID: covidwho-1450897
ABSTRACT

INTRODUCTION:

SARS-CoV-2 has been detected in various body fluids. Its presence in semen has been tested with contradictory results. This study aimed to detect the presence of SARS-CoV-2 virus using the real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) in semen and seminal plasma from men with active COVID-19 infection.

METHODS:

In a cross-sectional study at a COVID facility, men aged 20-45 years with active COVID-19 infection provided semen samples within 7 days of symptom onset or 5 days of nasopharyngeal rRT-PCR test positivity in asymptomatic men. Testing of SARS-CoV-2 was performed using rRT-PCR and semen analysis was done for sperm counts and motility as per the WHO (2010) standards.

RESULTS:

A total of 37 men with a mean age of 32.2 ± 5.6 years were tested. SARS CoV-2 virus could not be isolated in any of the samples. Further, microscopic analysis done on 17 samples showed normal semen parameters during the active phase of disease.

CONCLUSION:

Men with mild COVID-19 disease or asymptomatic individuals do not shed virus in their semen, ruling out sexual contact as a mode of transmission in this subset of population.

Full text: Available Collection: International databases Database: MEDLINE Type of study: Observational study / Randomized controlled trials Language: English Journal: Indian J Urol Year: 2021 Document Type: Article Affiliation country: Iju.iju_117_21

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Observational study / Randomized controlled trials Language: English Journal: Indian J Urol Year: 2021 Document Type: Article Affiliation country: Iju.iju_117_21