Status of contamination with SARS-CoV-2 in isolation wards of Huoshenshan hospital

ABSTRACT

OBJECTIVE: To evaluate the status of contamination with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) in isolation wards of Huoshenshan Hospital so as to provide scientific basis for developing reasonable strategies of management of environmental health in isolation wards of COVID-19. METHODS: From Mar 9, 2020 to Mar 29, 2020, the samples were systematically collected from 2 common isolation wards that were randomly selected from Huoshenshan Hospital. The sampling objects included environmental samples, samples from personal protective equipment(PPE) of medical staff and samples from hands of medical staff. The samples were collected by cotton swab wiping method, SARS-CoV-2 nucleic acid testing was performed by real time fluorescence quantitative polymerase chain reaction, and the testing results were colleted and analyzed. RESULTS: A total of 244 samples were collected. SARS-CoV-2 nucleic acid testing showed that 1 sample that was collected from bedrail of a patient was tested positive among 171 environmental samples, with the positive rate 0.58%;all of the samples that were collected from PPE of 49 medical staff were tested negative;1 sample that was collected from the hands of a patient was tested positive among 24 samples that were collected from hands of patients and medical staff, with the positive rate 4.17%. CONCLUSION: It is an effective way to reasonably plan for layout of isolation ward and isolation procedure and rigidly implement the systems of environmental disinfection and hand hygiene so as to reduce the degree of contamination with SARS-CoV-2 in isolation wards, cut off the transmission routes of the virus and reduce the risk of exposure to nosocomial infection, meanwhile, it is necessary to strengthen the personal hygiene management during prevention and control of COVID-19.