Transcriptomic analysis revealed increased expression of genes involved in keratinization in the tears of COVID-19 patients.
Sci Rep
; 11(1): 19817, 2021 10 06.
Article
in English
| MEDLINE | ID: covidwho-1454815
ABSTRACT
Recent studies have focused their attention on conjunctivitis as one of the symptoms of coronavirus disease 2019 (COVID-19). Therefore, tear samples were taken from COVID-19 patients and the presence of SARS-CoV-2 was evidenced using Real Time reverse transcription polymerase chain reaction. The main aim of this study was to analyze mRNA expression in the tears of patients with COVID-19 compared with healthy subjects using Next Generation Sequencing (NGS). The functional evaluation of the transcriptome highlighted 25 genes that differ statistically between healthy individuals and patients affected by COVID-19. In particular, the NGS analysis identified the presence of several genes involved in B cell signaling and keratinization. In particular, the genes involved in B cell signaling were downregulated in the tears of COVID-19 patients, while those involved in keratinization were upregulated. The results indicated that SARS-CoV-2 may induce a process of ocular keratinization and a defective B cell response.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Tears
/
Eye Diseases
/
Transcriptome
/
COVID-19
Type of study:
Experimental Studies
Limits:
Aged
/
Female
/
Humans
/
Male
Language:
English
Journal:
Sci Rep
Year:
2021
Document Type:
Article
Affiliation country:
S41598-021-99344-3
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