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Rapid field determination of SARS-CoV-2 by a colorimetric and fluorescent dual-functional lateral flow immunoassay biosensor.
Han, Han; Wang, Chongwen; Yang, Xingsheng; Zheng, Shuai; Cheng, Xiaodan; Liu, Zhenzhen; Zhao, Baohua; Xiao, Rui.
  • Han H; College of Life Science, Hebei Normal University, Shijiazhuang 050024, PR China.
  • Wang C; Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
  • Yang X; Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
  • Zheng S; College of Life Sciences, Anhui Agricultural University, Hefei 230036, PR China.
  • Cheng X; Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
  • Liu Z; Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
  • Zhao B; College of Life Sciences, Anhui Agricultural University, Hefei 230036, PR China.
  • Xiao R; Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
Sens Actuators B Chem ; 351: 130897, 2022 Jan 15.
Article in English | MEDLINE | ID: covidwho-1458630
ABSTRACT
The rapid and accurate diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at the early stage of virus infection can effectively prevent the spread of the virus and control the epidemic. Here, a colorimetric and fluorescent dual-functional lateral flow immunoassay (LFIA) biosensor was developed for the rapid and sensitive detection of spike 1 (S1) protein of SARS-CoV-2. A novel dual-functional immune label was fabricated by coating a single-layer shell formed by mixing 20 nm Au nanoparticles (Au NPs) and quantum dots (QDs) on SiO2 core to produce strong colorimetric and fluorescence signals and ensure good monodispersity and high stability. The colorimetric signal was used for visual detection and rapid screening of suspected SARS-CoV-2 infection on sites. The fluorescence signal was utilized for sensitive and quantitative detection of virus infection at the early stage. The detection limits of detecting S1 protein via colorimetric and fluorescence functions of the biosensor were 1 and 0.033 ng/mL, respectively. Furthermore, we evaluated the performance of the biosensor for analyzing real samples. The novel biosensor developed herein had good repeatability, specificity and accuracy, which showed great potential as a tool for rapidly detecting SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Experimental Studies Language: English Journal: Sens Actuators B Chem Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Experimental Studies Language: English Journal: Sens Actuators B Chem Year: 2022 Document Type: Article