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The oral vaccine based on self-replicating RNA lipid nanoparticles can simultaneously neutralize both SARS-CoV-2 variants alpha and delta.
Mohammadi, Golamabbas; Sotoudehnia Koranni, Zahra; Jebali, Ali.
  • Mohammadi G; Sirjan School of Medical Sciences, Sirjan, Iran.
  • Sotoudehnia Koranni Z; Medical Education Development Center, Sirjan School of Medical Sciences, Sirjan, Iran.
  • Jebali A; Department of Medical Nanotechnology, Faculty of Advanced Sciences and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran. Electronic address: alijebal2011@gmail.com.
Int Immunopharmacol ; 101(Pt B): 108231, 2021 Dec.
Article in English | MEDLINE | ID: covidwho-1458727
ABSTRACT
The aim of this study was to evaluate self-replicating RNA lipid nanoparticles (saRNA LNPs) to neutralize SARS-CoV-2 variants delta (B.1.617 lineage) and alpha (B.1.1.7 lineage). Before immunization of mice with saRNA LNPs, we saw high expression of S-protein at both mRNA and protein levels after transfection of HEK293T/17 cells with saRNA LNPs. After oral immunization of BALB/c mice with 0.1 - 10 µg saRNA LNPs , a high quantity of SARS-CoV-2 specific IgG and IgA antibodies were seen with a dose-dependent pattern. Importantly, the ratio of IgG2a/IgG1 in serum of vaccinated mice showed Th1/Th2 skewing response. We also found that the secreted antibodies could neutralize SARS-CoV-2 variants delta (B.1.617 lineage) and alpha (B.1.1.7 lineage). Re-stimulated splenocytes of vaccinated mice showed high secretion of IFN-γ, IL-6, and TNF- α . The authors think that although the preclinical study confirmed the efficacy of saRNA LNPs against SARS-CoV-2, the actual efficacy and safety of the oral vaccine must be evaluated in clinical trials.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA / Nanoparticles / COVID-19 Vaccines / SARS-CoV-2 / COVID-19 / Liposomes Type of study: Experimental Studies / Prognostic study Topics: Vaccines / Variants Limits: Animals / Humans Language: English Journal: Int Immunopharmacol Journal subject: Allergy and Immunology / Pharmacology Year: 2021 Document Type: Article Affiliation country: J.intimp.2021.108231

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA / Nanoparticles / COVID-19 Vaccines / SARS-CoV-2 / COVID-19 / Liposomes Type of study: Experimental Studies / Prognostic study Topics: Vaccines / Variants Limits: Animals / Humans Language: English Journal: Int Immunopharmacol Journal subject: Allergy and Immunology / Pharmacology Year: 2021 Document Type: Article Affiliation country: J.intimp.2021.108231