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SARS-CoV-2 Multi-Antigen Serology Assay.
Mazhari, Ramin; Ruybal-Pesántez, Shazia; Angrisano, Fiona; Kiernan-Walker, Nicholas; Hyslop, Stephanie; Longley, Rhea J; Bourke, Caitlin; Chen, Catherine; Williamson, Deborah A; Robinson, Leanne J; Mueller, Ivo; Eriksson, Emily M.
  • Mazhari R; Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia.
  • Ruybal-Pesántez S; Department of Medical Biology, The University of Melbourne, Melbourne, VIC 3052, Australia.
  • Angrisano F; Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia.
  • Kiernan-Walker N; Department of Medical Biology, The University of Melbourne, Melbourne, VIC 3052, Australia.
  • Hyslop S; Vector-Borne Diseases and Tropical Public Health, Burnet Institute, Melbourne, VIC 3004, Australia.
  • Longley RJ; Vector-Borne Diseases and Tropical Public Health, Burnet Institute, Melbourne, VIC 3004, Australia.
  • Bourke C; Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia.
  • Chen C; Department of Medical Biology, The University of Melbourne, Melbourne, VIC 3052, Australia.
  • Williamson DA; Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia.
  • Robinson LJ; Department of Medical Biology, The University of Melbourne, Melbourne, VIC 3052, Australia.
  • Mueller I; Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia.
  • Eriksson EM; Department of Medical Biology, The University of Melbourne, Melbourne, VIC 3052, Australia.
Methods Protoc ; 4(4)2021 Oct 09.
Article in English | MEDLINE | ID: covidwho-1463776
ABSTRACT
Serology tests are extremely useful for assessing whether a person has been infected with a pathogen. Since the onset of the COVID-19 pandemic, measurement of anti-SARS-CoV-2-specific antibodies has been considered an essential tool in identifying seropositive individuals and thereby understanding the extent of transmission in communities. The Luminex system is a bead-based technology that has the capacity to assess multiple antigens simultaneously using very low sample volumes and is ideal for high-throughput studies. We have adapted this technology to develop a COVID-19 multi-antigen serological assay. This protocol described here carefully outlines recommended steps to optimize and establish this method for COVID-19-specific antibody measurement in plasma and in saliva. However, the protocol can easily be customized and thus the assay is broadly applicable to measure antibodies to other pathogens.
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Full text: Available Collection: International databases Database: MEDLINE Language: English Year: 2021 Document Type: Article Affiliation country: Mps4040072

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Full text: Available Collection: International databases Database: MEDLINE Language: English Year: 2021 Document Type: Article Affiliation country: Mps4040072