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Rapid diagnostic methods for SARS-CoV-2 (COVID-19) detection: an evidence-based report.
Verma, Manish Kumar; Sharma, Parshant Kumar; Verma, Henu Kumar; Singh, Anand Narayan; Singh, Desh Deepak; Verma, Poonam; Siddiqui, Areena Hoda.
  • Verma MK; Department of Biochemistry, G.S.V.M. Medical College, Kanpur, India.
  • Sharma PK; Department of Electronic Engineering, Kwangwoon University, Nowon-gu, Seoul, South Korea.
  • Verma HK; Department of Immunopathology, Institute of lungs Biology and Disease, Comprehensive Pneumology Center, Munich, Germany.
  • Singh AN; Department of Biochemistry, G.S.V.M. Medical College, Kanpur, India.
  • Singh DD; Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur, India.
  • Verma P; Department of Biotechnology, IFTM University, Moradabad, India.
  • Siddiqui AH; Department of Laboratory Medicine, Sahara Hospital, Viraj Khand, Gomti Nagar, Lucknow, India.
J Med Life ; 14(4): 431-442, 2021.
Article in English | MEDLINE | ID: covidwho-1464171
ABSTRACT
Since December 2019, the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been a global health concern. The transmission method is human-to-human. Since this second wave of SARS-CoV-2 is more aggressive than the first wave, rapid testing is warranted to use practical diagnostics to break the transfer chain. Currently, various techniques are used to diagnose SARS-CoV-2 infection, each with its own set of advantages and disadvantages. A full review of online databases such as PubMed, EMBASE, Web of Science, and Google Scholar was analyzed to identify relevant articles focusing on SARS-CoV-2 and diagnosis and therapeutics. The most recent article search was on May 10, 2021. We summarize promising methods for detecting the novel Coronavirus using sensor-based diagnostic technologies that are sensitive, cost-effective, and simple to use at the point of care. This includes loop-mediated isothermal amplification and several laboratory protocols for confirming suspected 2019-nCoV cases, as well as studies with non-commercial laboratory protocols based on real-time reverse transcription-polymerase chain reaction and a field-effect transistor-based bio-sensing device. We discuss a potential discovery that could lead to the mass and targeted SARS-CoV-2 detection needed to manage the COVID-19 pandemic through infection succession and timely therapy.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Clinical Practice Guide / Evidence synthesis / Prognostic study / Reviews Limits: Humans Language: English Journal: J Med Life Journal subject: Medicine Year: 2021 Document Type: Article Affiliation country: Jml-2021-0168

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Clinical Practice Guide / Evidence synthesis / Prognostic study / Reviews Limits: Humans Language: English Journal: J Med Life Journal subject: Medicine Year: 2021 Document Type: Article Affiliation country: Jml-2021-0168