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Replication and single-cycle delivery of SARS-CoV-2 replicons.
Ricardo-Lax, Inna; Luna, Joseph M; Thao, Tran Thi Nhu; Le Pen, Jérémie; Yu, Yingpu; Hoffmann, H-Heinrich; Schneider, William M; Razooky, Brandon S; Fernandez-Martinez, Javier; Schmidt, Fabian; Weisblum, Yiska; Trüeb, Bettina Salome; Berenguer Veiga, Inês; Schmied, Kimberly; Ebert, Nadine; Michailidis, Eleftherios; Peace, Avery; Sánchez-Rivera, Francisco J; Lowe, Scott W; Rout, Michael P; Hatziioannou, Theodora; Bieniasz, Paul D; Poirier, John T; MacDonald, Margaret R; Thiel, Volker; Rice, Charles M.
  • Ricardo-Lax I; Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
  • Luna JM; Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
  • Thao TTN; Institute of Virology and Immunology (IVI), Bern, Switzerland.
  • Le Pen J; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Yu Y; Graduate School for Biomedical Science, University of Bern, Bern, Switzerland.
  • Hoffmann HH; Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
  • Schneider WM; Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
  • Razooky BS; Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
  • Fernandez-Martinez J; Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
  • Schmidt F; Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
  • Weisblum Y; Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, NY 10065, USA.
  • Trüeb BS; Laboratory of Retrovirology, The Rockefeller University, New York, NY 10065, USA.
  • Berenguer Veiga I; Laboratory of Retrovirology, The Rockefeller University, New York, NY 10065, USA.
  • Schmied K; Institute of Virology and Immunology (IVI), Bern, Switzerland.
  • Ebert N; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Michailidis E; Institute of Virology and Immunology (IVI), Bern, Switzerland.
  • Peace A; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Sánchez-Rivera FJ; Institute of Virology and Immunology (IVI), Bern, Switzerland.
  • Lowe SW; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Rout MP; Institute of Virology and Immunology (IVI), Bern, Switzerland.
  • Hatziioannou T; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Bieniasz PD; Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
  • Poirier JT; Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
  • MacDonald MR; Cancer Biology and Genetics, MSKCC, New York, NY 10065, USA.
  • Thiel V; Cancer Biology and Genetics, MSKCC, New York, NY 10065, USA.
  • Rice CM; Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, NY 10065, USA.
Science ; 374(6571): 1099-1106, 2021 Nov 26.
Article in English | MEDLINE | ID: covidwho-1467657
ABSTRACT
Molecular virology tools are critical for basic studies of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental systems that do not rely on viruses capable of spread are needed for potential use in lower-containment settings. In this work, we use a yeast-based reverse genetics system to develop spike-deleted SARS-CoV-2 self-replicating RNAs. These noninfectious self-replicating RNAs, or replicons, can be trans-complemented with viral glycoproteins to generate replicon delivery particles for single-cycle delivery into a range of cell types. This SARS-CoV-2 replicon system represents a convenient and versatile platform for antiviral drug screening, neutralization assays, host factor validation, and viral variant characterization.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Replicon / RNA, Viral / SARS-CoV-2 Type of study: Prognostic study Topics: Variants Limits: Animals / Humans Language: English Journal: Science Year: 2021 Document Type: Article Affiliation country: Science.abj8430

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Replicon / RNA, Viral / SARS-CoV-2 Type of study: Prognostic study Topics: Variants Limits: Animals / Humans Language: English Journal: Science Year: 2021 Document Type: Article Affiliation country: Science.abj8430