Replication and single-cycle delivery of SARS-CoV-2 replicons.
Science
; 374(6571): 1099-1106, 2021 Nov 26.
Article
in English
| MEDLINE | ID: covidwho-1467657
ABSTRACT
Molecular virology tools are critical for basic studies of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental systems that do not rely on viruses capable of spread are needed for potential use in lower-containment settings. In this work, we use a yeast-based reverse genetics system to develop spike-deleted SARS-CoV-2 self-replicating RNAs. These noninfectious self-replicating RNAs, or replicons, can be trans-complemented with viral glycoproteins to generate replicon delivery particles for single-cycle delivery into a range of cell types. This SARS-CoV-2 replicon system represents a convenient and versatile platform for antiviral drug screening, neutralization assays, host factor validation, and viral variant characterization.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Replicon
/
RNA, Viral
/
SARS-CoV-2
Type of study:
Prognostic study
Topics:
Variants
Limits:
Animals
/
Humans
Language:
English
Journal:
Science
Year:
2021
Document Type:
Article
Affiliation country:
Science.abj8430
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