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Methods to Measure Antibody Neutralization of Live Human Coronavirus OC43.
Boonyaratanakornkit, Jim; Sholukh, Anton M; Gray, Matthew; Bossard, Emily L; Ford, Emily S; Corbett, Kizzmekia S; Corey, Lawrence; Taylor, Justin J.
  • Boonyaratanakornkit J; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.
  • Sholukh AM; Department of Medicine, University of Washington, Seattle, WA 98195, USA.
  • Gray M; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.
  • Bossard EL; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.
  • Ford ES; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.
  • Corbett KS; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.
  • Corey L; Department of Medicine, University of Washington, Seattle, WA 98195, USA.
  • Taylor JJ; Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA.
Viruses ; 13(10)2021 10 14.
Article in English | MEDLINE | ID: covidwho-1469383
ABSTRACT
The human Betacoronavirus OC43 is a common cause of respiratory viral infections in adults and children. Lung infections with OC43 are associated with mortality, especially in hematopoietic stem cell transplant recipients. Neutralizing antibodies play a major role in protection against many respiratory viral infections, but to date a live viral neutralization assay for OC43 has not been described. We isolated a human monoclonal antibody (OC2) that binds to the spike protein of OC43 and neutralizes the live virus derived from the original isolate of OC43. We used this monoclonal antibody to develop and test the performance of two readily accessible in vitro assays for measuring antibody neutralization, one utilizing cytopathic effect and another utilizing an ELISA of infected cells. We used both methods to measure the neutralizing activity of the OC2 monoclonal antibody and of human plasma. These assays could prove useful for studying humoral responses to OC43 and cross-neutralization with other medically important betacoronaviruses.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Neutralization Tests / Coronavirus OC43, Human / Antibodies, Neutralizing / Spike Glycoprotein, Coronavirus / Antibodies, Monoclonal / Antibodies, Viral Type of study: Randomized controlled trials Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: V13102075

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Neutralization Tests / Coronavirus OC43, Human / Antibodies, Neutralizing / Spike Glycoprotein, Coronavirus / Antibodies, Monoclonal / Antibodies, Viral Type of study: Randomized controlled trials Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: V13102075