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Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads.
Conzentino, Marcelo S; Santos, Tatielle P C; Selim, Khaled A; Wagner, Berenike; Alford, Janette T; Deobald, Nelli; Paula, Nigela M; Rego, Fabiane G M; Zanette, Dalila L; Aoki, Mateus N; Nardin, Jeanine M; Huergo, Maria C C; Reis, Rodrigo A; Huergo, Luciano F.
  • Conzentino MS; Setor Litoral, UFPR Matinhos, PR, Brazil.
  • Santos TPC; Setor Litoral, UFPR Matinhos, PR, Brazil.
  • Selim KA; Organismic Interactions Department, Interfaculty Institute for Microbiology and Infection Medicine, Cluster of Excellence 'Controlling Microbes to Fight Infections', Tübingen University, Auf der Morgenstelle 28, 72076, Tübingen, Germany.
  • Wagner B; Organismic Interactions Department, Interfaculty Institute for Microbiology and Infection Medicine, Cluster of Excellence 'Controlling Microbes to Fight Infections', Tübingen University, Auf der Morgenstelle 28, 72076, Tübingen, Germany.
  • Alford JT; Organismic Interactions Department, Interfaculty Institute for Microbiology and Infection Medicine, Cluster of Excellence 'Controlling Microbes to Fight Infections', Tübingen University, Auf der Morgenstelle 28, 72076, Tübingen, Germany.
  • Deobald N; Organismic Interactions Department, Interfaculty Institute for Microbiology and Infection Medicine, Cluster of Excellence 'Controlling Microbes to Fight Infections', Tübingen University, Auf der Morgenstelle 28, 72076, Tübingen, Germany.
  • Paula NM; Setor Litoral, UFPR Matinhos, PR, Brazil.
  • Rego FGM; Post-Graduation Program in Pharmaceutical Sciences, UFPR, Curitiba, PR, Brazil.
  • Zanette DL; Instituto Carlos Chagas, FioCruz, PR, Brazil.
  • Aoki MN; Instituto Carlos Chagas, FioCruz, PR, Brazil.
  • Nardin JM; Hospital Erasto Gaertner, Curitiba, PR, Brazil.
  • Huergo MCC; Setor Litoral, UFPR Matinhos, PR, Brazil.
  • Reis RA; Department of Cell Biology, UFPR, Curitiba, PR, Brazil.
  • Huergo LF; Setor Litoral, UFPR Matinhos, PR, Brazil. Electronic address: luciano.huergo@gmail.com.
Anal Biochem ; 631: 114360, 2021 10 15.
Article in English | MEDLINE | ID: covidwho-1474246
ABSTRACT
To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni2+ magnetic particles coated with His tagged SARS-CoV-2 antigens. A simple and inexpensive 96 well plate magnetic extraction/homogenization process is described which allows the simultaneous analysis of 96 samples and delivers results in 7 min with high accuracy.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoglobulin G / COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study Limits: Humans Language: English Journal: Anal Biochem Year: 2021 Document Type: Article Affiliation country: J.ab.2021.114360

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoglobulin G / COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study Limits: Humans Language: English Journal: Anal Biochem Year: 2021 Document Type: Article Affiliation country: J.ab.2021.114360