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Equipment-free, salt-mediated immobilization of nucleic acids for nucleic acid lateral flow assays.
Park, Jung Soo; Kim, Seokjoon; Han, Jinjoo; Kim, Jung Ho; Park, Ki Soo.
  • Park JS; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
  • Kim S; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
  • Han J; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
  • Kim JH; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
  • Park KS; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
Sens Actuators B Chem ; 351: 130975, 2022 Jan 15.
Article in English | MEDLINE | ID: covidwho-1475063
ABSTRACT
As the world has been facing several deadly virus crises, including Zika virus disease, Ebola virus disease, severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and Coronavirus disease 2019 (COVID-19), lateral flow assays (LFAs), which require minimal equipment for point-of-care of viral infectious diseases, are garnering much attention. Accordingly, there is an increasing demand to reduce the time and cost required for manufacturing LFAs. The current study introduces an equipment-free method of salt-mediated immobilization of nucleic acids (SAIoNs) for LFAs. Compared to general DNA immobilization methods such as streptavidin-biotin, UV-irradiation, and heat treatment, our method does not require special equipment (e.g., centrifuge, UV-crosslinker, heating device); therefore, it can be applied in a resource-limited environment with reduced production costs. The immobilization process was streamlined and completed within 30 min. Our method improved the color intensity signal approximately 14 times compared to the method without using SAIoNs and exhibited reproducibility with the long-term storage stability. The proposed method can be used to detect practical targets (e.g., SARS-CoV-2) and facilitates highly sensitive and selective detection of target nucleic acids with multiplexing capability and without any cross-reactivity. This novel immobilization strategy provides a basis for easily and inexpensively developing nucleic acid LFAs combined with various types of nucleic acid amplification.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Randomized controlled trials Language: English Journal: Sens Actuators B Chem Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Randomized controlled trials Language: English Journal: Sens Actuators B Chem Year: 2022 Document Type: Article