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Lateral flow assays (LFA) as an alternative medical diagnosis method for detection of virus species: The intertwine of nanotechnology with sensing strategies.
Sadeghi, Poorya; Sohrabi, Hessamaddin; Hejazi, Maryam; Jahanban-Esfahlan, Ali; Baradaran, Behzad; Tohidast, Maryam; Majidi, Mir Reza; Mokhtarzadeh, Ahad; Tavangar, Seyed Mohammad; de la Guardia, Miguel.
  • Sadeghi P; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Sohrabi H; Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
  • Hejazi M; Chronic Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
  • Jahanban-Esfahlan A; Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Baradaran B; Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Tohidast M; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Majidi MR; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Mokhtarzadeh A; Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
  • Tavangar SM; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • de la Guardia M; Department of Pathology, Dr. Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Trends Analyt Chem ; 145: 116460, 2021 Dec.
Article in English | MEDLINE | ID: covidwho-1475093
ABSTRACT
Viruses are responsible for multiple infections in humans that impose huge health burdens on individuals and populations worldwide. Therefore, numerous diagnostic methods and strategies have been developed for prevention, management, and decreasing the burden of viral diseases, each having its advantages and limitations. Viral infections are commonly detected using serological and nucleic acid-based methods. However, these conventional and clinical approaches have some limitations that can be resolved by implementing other detector devices. Therefore, the search for sensitive, selective, portable, and costless approaches as efficient alternative clinical methods for point of care testing (POCT) analysis has gained much attention in recent years. POCT is one of the ultimate goals in virus detection, and thus, the tests need to be rapid, specific, sensitive, accessible, and user-friendly. In this review, after a brief overview of viruses and their characteristics, the conventional viral detection methods, the clinical approaches, and their advantages and shortcomings are firstly explained. Then, LFA systems working principles, benefits, classification are discussed. Furthermore, the studies regarding designing and employing LFAs in diagnosing different types of viruses, especially SARS-CoV-2 as a main concern worldwide and innovations in the LFAs' approaches and designs, are comprehensively discussed here. Furthermore, several strategies addressed in some studies for overcoming LFA limitations like low sensitivity are reviewed. Numerous techniques are adopted to increase sensitivity and perform quantitative detection. Employing several visualization methods, using different labeling reporters, integrating LFAs with other detection methods to benefit from both LFA and the integrated detection device advantages, and designing unique membranes to increase reagent reactivity, are some of the approaches that are highlighted.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Prognostic study Language: English Journal: Trends Analyt Chem Year: 2021 Document Type: Article Affiliation country: J.trac.2021.116460

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Prognostic study Language: English Journal: Trends Analyt Chem Year: 2021 Document Type: Article Affiliation country: J.trac.2021.116460