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SARS-CoV-2 Antibody Binding and Neutralization in Dried Blood Spot Eluates and Paired Plasma.
Itell, Hannah L; Weight, Haidyn; Fish, Carolyn S; Logue, Jennifer K; Franko, Nicholas; Wolf, Caitlin R; McCulloch, Denise J; Galloway, Jared; Matsen, Frederick A; Chu, Helen Y; Overbaugh, Julie.
  • Itell HL; Human Biology Division, Fred Hutchinson Cancer Research Centergrid.270240.3, Seattle, Washington, USA.
  • Weight H; Molecular and Cellular Biology Graduate Program, University of Washingtongrid.34477.33 and Fred Hutchinson Cancer Research Centergrid.270240.3, Seattle, Washington, USA.
  • Fish CS; Human Biology Division, Fred Hutchinson Cancer Research Centergrid.270240.3, Seattle, Washington, USA.
  • Logue JK; Human Biology Division, Fred Hutchinson Cancer Research Centergrid.270240.3, Seattle, Washington, USA.
  • Franko N; Department of Medicine, University of Washingtongrid.34477.33, Seattle, Washington, USA.
  • Wolf CR; Department of Medicine, University of Washingtongrid.34477.33, Seattle, Washington, USA.
  • McCulloch DJ; Department of Medicine, University of Washingtongrid.34477.33, Seattle, Washington, USA.
  • Galloway J; Department of Medicine, University of Washingtongrid.34477.33, Seattle, Washington, USA.
  • Matsen FA; Public Health Sciences Division, Fred Hutchinson Cancer Research Centergrid.270240.3, Seattle, Washington, USA.
  • Chu HY; Public Health Sciences Division, Fred Hutchinson Cancer Research Centergrid.270240.3, Seattle, Washington, USA.
  • Overbaugh J; Department of Medicine, University of Washingtongrid.34477.33, Seattle, Washington, USA.
Microbiol Spectr ; 9(2): e0129821, 2021 10 31.
Article in English | MEDLINE | ID: covidwho-1476403
Preprint
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ABSTRACT
Wide-scale assessment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific antibodies is critical to understanding population seroprevalence, correlates of protection, and the longevity of vaccine-elicited responses. Most SARS-CoV-2 studies characterize antibody responses in plasma/sera. While reliable and broadly used, these samples pose several logistical restrictions, such as requiring venipuncture for collection and a cold chain for transportation and storage. Dried blood spots (DBS) overcome these barriers as they can be self-collected by fingerstick and mailed and stored at ambient temperature. Here, we evaluate the suitability of DBS for SARS-CoV-2 antibody assays by comparing several antibody responses between paired plasma and DBS from SARS-CoV-2 convalescent and vaccinated individuals. We found that DBS not only reflected plasma antibody binding by enzyme-linked immunosorbent assay (ELISA) and epitope profiles using phage display, but also yielded SARS-CoV-2 neutralization titers that highly correlated with paired plasma. Neutralization measurement was further streamlined by adapting assays to a high-throughput 384-well format. This study supports the adoption of DBS for numerous SARS-CoV-2 binding and neutralization assays. IMPORTANCE Plasma and sera isolated from venous blood represent conventional sample types used for the evaluation of SARS-CoV-2 antibody responses after infection or vaccination. However, collection of these samples is invasive and requires trained personnel and equipment for immediate processing. Once collected, plasma and sera must be stored and shipped at cold temperatures. To define the risk of emerging SARS-CoV-2 variants and the longevity of immune responses to natural infection and vaccination, it will be necessary to measure various antibody features in populations around the world, including in resource-limited areas. A sampling method that is compatible with these settings and is suitable for a variety of SARS-CoV-2 antibody assays is therefore needed to continue to understand and curb the COVID-19 pandemic.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antibodies, Neutralizing / Dried Blood Spot Testing / COVID-19 Serological Testing / COVID-19 / Antibodies, Viral Type of study: Diagnostic study / Experimental Studies / Observational study / Prognostic study Topics: Vaccines / Variants Limits: Humans Language: English Journal: Microbiol Spectr Year: 2021 Document Type: Article Affiliation country: Spectrum.01298-21

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antibodies, Neutralizing / Dried Blood Spot Testing / COVID-19 Serological Testing / COVID-19 / Antibodies, Viral Type of study: Diagnostic study / Experimental Studies / Observational study / Prognostic study Topics: Vaccines / Variants Limits: Humans Language: English Journal: Microbiol Spectr Year: 2021 Document Type: Article Affiliation country: Spectrum.01298-21