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Phytochemical Profile, Free Radical Scavenging and Anti-Inflammatory Properties of Acalypha Indica Root Extract: Evidence from In Vitro and In Vivo Studies.
Sahukari, Ravi; Punabaka, Jyothi; Bhasha, Shanmugam; Ganjikunta, Venkata Subbaiah; Kondeti Ramudu, Shanmugam; Kesireddy, Sathyavelu Reddy; Ye, Weibing; Korivi, Mallikarjuna.
  • Sahukari R; Department of Zoology, Sri Venkateswara University, Tirupati 517502, India.
  • Punabaka J; Department of Zoology, Sri Venkateswara University, Tirupati 517502, India.
  • Bhasha S; Department of Zoology, Sri Venkateswara University, Tirupati 517502, India.
  • Ganjikunta VS; Department of Zoology, Sri Venkateswara University, Tirupati 517502, India.
  • Kondeti Ramudu S; Department of Zoology, PRR &VS Government Degree College, Vidavalur 524318, India.
  • Kesireddy SR; Department of Zoology, Sri Venkateswara University, Tirupati 517502, India.
  • Ye W; Exercise and Metabolism Research Center, College of Physical Education and Health Sciences, Zhejiang Normal University, Jinhua 321004, China.
  • Korivi M; Exercise and Metabolism Research Center, College of Physical Education and Health Sciences, Zhejiang Normal University, Jinhua 321004, China.
Molecules ; 26(20)2021 Oct 15.
Article in English | MEDLINE | ID: covidwho-1480885
ABSTRACT
In our in vitro and in vivo studies, we used Acalypha indica root methanolic extract (AIRME), and investigated their free radical scavenging/antioxidant and anti-inflammatory properties. Primarily, phytochemical analysis showed rich content of phenols (70.92 mg of gallic acid/g) and flavonoids (16.01 mg of rutin/g) in AIRME. We then performed HR-LC-MS and GC-MS analyses, and identified 101 and 14 phytochemical compounds, respectively. Among them, ramipril glucuronide (1.563%), antimycin A (1.324%), swietenine (1.134%), quinone (1.152%), oxprenolol (1.118%), choline (0.847%), bumetanide (0.847%) and fenofibrate (0.711%) are the predominant phytomolecules. Evidence from in vitro studies revealed that AIRME scavenges DPPH and hydroxyl radicals in a concentration dependent manner (10-50 µg/mL). Similarly, hydrogen peroxide and lipid peroxidation were also remarkably inhibited by AIRME as concentration increases (20-100 µg/mL). In vitro antioxidant activity of AIRME was comparable to ascorbic acid treatment. For in vivo studies, carrageenan (1%, sub-plantar) was injected to rats to induce localized inflammation. Acute inflammation was represented by paw-edema, and significantly elevated (p < 0.05) WBC, platelets and C-reactive protein (CRP). However, AIRME pretreatment (150/300 mg/kg bodyweight) significantly (p < 0.05) decreased edema volume. This was accompanied by a significant (p < 0.05) reduction of WBC, platelets and CRP with both doses of AIRME. The decreased activities of superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase in paw tissue were restored (p < 0.05 / p < 0.01) with AIRME in a dose-dependent manner. Furthermore, AIRME attenuated carrageenan-induced neutrophil infiltrations and vascular dilation in paw tissue. For the first time, our findings demonstrated the potent antioxidant and anti-inflammatory properties of AIRME, which could be considered to develop novel anti-inflammatory drugs.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Plants, Medicinal / Phytochemicals / Acalypha Topics: Traditional medicine Limits: Animals Language: English Journal subject: Biology Year: 2021 Document Type: Article Affiliation country: Molecules26206251

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Plants, Medicinal / Phytochemicals / Acalypha Topics: Traditional medicine Limits: Animals Language: English Journal subject: Biology Year: 2021 Document Type: Article Affiliation country: Molecules26206251