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Isolation of Primary Human and Rodent Brain Microvascular Endothelial Cells: Culturing, Characterization, and High-Efficiency Transfection.
Darbinian, Nune; Darbinyan, Armine; Merabova, Nana; Amini, Shohreh.
  • Darbinian N; Department of Neurology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA. nsarkiss@temple.edu.
  • Darbinyan A; Shriners Hospital Pediatric Research Center, Temple University School of Medicine, Philadelphia, PA, USA. nsarkiss@temple.edu.
  • Merabova N; Department of Pathology, Yale University School of Medicine, New Haven, CT, USA.
  • Amini S; Shriners Hospital Pediatric Research Center, Temple University School of Medicine, Philadelphia, PA, USA.
Methods Mol Biol ; 2311: 185-193, 2021.
Article in English | MEDLINE | ID: covidwho-1482181
ABSTRACT
Studies of blood-brain barrier (BBB) require developing of a novel and convenient in vitro endothelial cell model. We isolated primary human and rodent brain microvascular endothelial cells and developed methods for culturing, characterization, and high-efficiency transfection of endothelial cells. Here, we describe the improved methods to obtain in vitro human and rodent BBB models to study expression of endogenous and exogenous genes of interest.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Brain / Blood-Brain Barrier / Transfection / Cell Separation / Endothelial Cells / Microvessels Limits: Animals / Humans Language: English Journal: Methods Mol Biol Journal subject: Molecular Biology Year: 2021 Document Type: Article Affiliation country: 978-1-0716-1437-2_15

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Brain / Blood-Brain Barrier / Transfection / Cell Separation / Endothelial Cells / Microvessels Limits: Animals / Humans Language: English Journal: Methods Mol Biol Journal subject: Molecular Biology Year: 2021 Document Type: Article Affiliation country: 978-1-0716-1437-2_15