Single-cell profiling of proteins and chromatin accessibility using PHAGE-ATAC.
Nat Biotechnol
; 40(3): 374-381, 2022 03.
Article
in English
| MEDLINE | ID: covidwho-1483138
ABSTRACT
Multimodal measurements of single-cell profiles are proving increasingly useful for characterizing cell states and regulatory mechanisms. In the present study, we developed PHAGE-ATAC (Assay for Transposase-Accessible Chromatin), a massively parallel droplet-based method that uses phage displaying, engineered, camelid single-domain antibodies ('nanobodies') for simultaneous single-cell measurements of protein levels and chromatin accessibility profiles, and mitochondrial DNA-based clonal tracing. We use PHAGE-ATAC for multimodal analysis in primary human immune cells, sample multiplexing, intracellular protein analysis and the detection of SARS-CoV-2 spike protein in human cell populations. Finally, we construct a synthetic high-complexity phage library for selection of antigen-specific nanobodies that bind cells of particular molecular profiles, opening an avenue for protein detection, cell characterization and screening with single-cell genomics.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Bacteriophages
/
COVID-19
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Nat Biotechnol
Journal subject:
Biotechnology
Year:
2022
Document Type:
Article
Affiliation country:
S41587-021-01065-5
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