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Factors that Influence the Reported Sensitivity of Rapid Antigen Testing for SARS-CoV-2.
Parvu, Valentin; Gary, Devin S; Mann, Joseph; Lin, Yu-Chih; Mills, Dorsey; Cooper, Lauren; Andrews, Jeffrey C; Manabe, Yukari C; Pekosz, Andrew; Cooper, Charles K.
  • Parvu V; Becton, Dickinson and Company, BD Life Sciences-Integrated Diagnostic Solutions, Sparks, MD, United States.
  • Gary DS; Becton, Dickinson and Company, BD Life Sciences-Integrated Diagnostic Solutions, Sparks, MD, United States.
  • Mann J; Becton, Dickinson and Company, BD Life Sciences-Integrated Diagnostic Solutions, Sparks, MD, United States.
  • Lin YC; Becton, Dickinson and Company, BD Life Sciences-Integrated Diagnostic Solutions, Sparks, MD, United States.
  • Mills D; Becton, Dickinson and Company, BD Life Sciences-Integrated Diagnostic Solutions, Sparks, MD, United States.
  • Cooper L; Becton, Dickinson and Company, BD Life Sciences-Integrated Diagnostic Solutions, Sparks, MD, United States.
  • Andrews JC; Becton, Dickinson and Company, BD Life Sciences-Integrated Diagnostic Solutions, Sparks, MD, United States.
  • Manabe YC; W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States.
  • Pekosz A; Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
  • Cooper CK; W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States.
Front Microbiol ; 12: 714242, 2021.
Article in English | MEDLINE | ID: covidwho-1485072
ABSTRACT
Tests that detect the presence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) antigen in clinical specimens from the upper respiratory tract can provide a rapid means of coronavirus disease 2019 (COVID-19) diagnosis and help identify individuals who may be infectious and should isolate to prevent SARS-CoV-2 transmission. This systematic review assesses the diagnostic accuracy of SARS-CoV-2 antigen detection in COVID-19 symptomatic and asymptomatic individuals compared to quantitative reverse transcription polymerase chain reaction (RT-qPCR) and summarizes antigen test sensitivity using meta-regression. In total, 83 studies were included that compared SARS-CoV-2 rapid antigen-based lateral flow testing (RALFT) to RT-qPCR for SARS-CoV-2. Generally, the quality of the evaluated studies was inconsistent; nevertheless, the overall sensitivity for RALFT was determined to be 75.0% (95% confidence interval 71.0-78.0). Additionally, RALFT sensitivity was found to be higher for symptomatic vs. asymptomatic individuals and was higher for a symptomatic population within 7 days from symptom onset compared to a population with extended days of symptoms. Viral load was found to be the most important factor for determining SARS-CoV-2 antigen test sensitivity. Other design factors, such as specimen storage and anatomical collection type, also affect the performance of RALFT. RALFT and RT-qPCR testing both achieve high sensitivity when compared to SARS-CoV-2 viral culture.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Experimental Studies / Prognostic study / Reviews / Systematic review/Meta Analysis Language: English Journal: Front Microbiol Year: 2021 Document Type: Article Affiliation country: Fmicb.2021.714242

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Experimental Studies / Prognostic study / Reviews / Systematic review/Meta Analysis Language: English Journal: Front Microbiol Year: 2021 Document Type: Article Affiliation country: Fmicb.2021.714242