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Label-free detection of SARS-CoV-2 Spike S1 antigen triggered by electroactive gold nanoparticles on antibody coated fluorine-doped tin oxide (FTO) electrode.
Roberts, Akanksha; Mahari, Subhasis; Shahdeo, Deepshikha; Gandhi, Sonu.
  • Roberts A; DBT-National Institute of Animal Biotechnology (NIAB), Hyderabad, 500032, Telangana, India.
  • Mahari S; DBT-National Institute of Animal Biotechnology (NIAB), Hyderabad, 500032, Telangana, India.
  • Shahdeo D; DBT-National Institute of Animal Biotechnology (NIAB), Hyderabad, 500032, Telangana, India.
  • Gandhi S; DBT-National Institute of Animal Biotechnology (NIAB), Hyderabad, 500032, Telangana, India. Electronic address: gandhi@niab.org.in.
Anal Chim Acta ; 1188: 339207, 2021 Dec 15.
Article in English | MEDLINE | ID: covidwho-1487555
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, also known as 2019-nCov or COVID-19) outbreak has become a huge public health issue due to its rapid transmission making it a global pandemic. Here, we report fabricated fluorine doped tin oxide (FTO) electrodes/gold nanoparticles (AuNPs) complex coupled with in-house developed SARS-CoV-2 spike S1 antibody (SARS-CoV-2 Ab) to measure the response with Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV). The biophysical characterisation of FTO/AuNPs/SARS-CoV-2Ab was done via UV-Visible spectroscopy, Dynamic Light Scattering (DLS), and Fourier Transform Infrared Spectroscopy (FT-IR). The fabricated FTO/AuNPs/SARS-CoV-2Ab immunosensor was optimised for response time, antibody concentration, temperature, and pH. Under optimum conditions, the FTO/AuNPs/Ab based immunosensor displayed high sensitivity with limit of detection (LOD) up to 0.63 fM in standard buffer and 120 fM in spiked saliva samples for detection of SARS-CoV-2 spike S1 antigen (Ag) with negligible cross reactivity Middle East Respiratory Syndrome (MERS) spike protein. The proposed FTO/AuNPs/SARS-CoV-2Ab based biosensor proved to be stable for up to 4 weeks and can be used as an alternative non-invasive diagnostic tool for the rapid, specific and sensitive detection of SARS-CoV-2 Spike Ag traces in clinical samples.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Metal Nanoparticles / Spike Glycoprotein, Coronavirus / COVID-19 Type of study: Diagnostic study / Randomized controlled trials Limits: Humans Language: English Journal: Anal Chim Acta Year: 2021 Document Type: Article Affiliation country: J.aca.2021.339207

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Metal Nanoparticles / Spike Glycoprotein, Coronavirus / COVID-19 Type of study: Diagnostic study / Randomized controlled trials Limits: Humans Language: English Journal: Anal Chim Acta Year: 2021 Document Type: Article Affiliation country: J.aca.2021.339207